Talk:20.109(F14): Mod 1 Day 7 FACS analysis: Difference between revisions

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Revision as of 06:56, 3 October 2014

Schedule

Sign up for a time to do flow cytometry in the table below. Please arrive at the time you choose and then expect to spend about 45 minutes in TC and another 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.

T/R

Time block Team 1 Team 2 Team 3
I: 1-2:30 pm Yellow Mustard Red Cherries Pink Bubblegum
II: 2-3:30-pm Blue team, best team Green Tiger Team!
III: 3-4:30 pm Purple team

W/F

Time block Team 1 Team 2
I: 1-2:30 pm Pinkle Green
II: 2-3:30-pm Blue


Data Analysis

Reported data

To aid class-wide comparisons (and statistics!), please post the requested information in the table below in a timely fashion using three significant digits (ex. 3.72). For samples done in triplicate, please separate the three values with a semi-colon. Once you've completed your statistics, don't get over-excited with the number of significant figures you report! For example, "3.7" will suffice. Please describe the experimental test conditions in the form "0.2 ug D5 and 0.05 ug CUT D3." Be sure to include which enzyme was used to cut D3.

T/R

Team color % R2 Gated Mock EGFP Δ5 uncut Δ3 cut Δ3 Δ5+uncut Δ3 Δ5+cut Δ3 Exp A Exp condition Exp B Exp condition
Cherries
Tiger
Mustard
Green
Blue, best
Bubblegum
Purple

W/F

Team color % R2 Gated Mock EGFP Δ5 uncut Δ3 cut Δ3 Δ5+uncut Δ3 Δ5+cut Δ3 Exp A Exp condition Exp B Exp condition
Pinkle
Blue
Green