Talk:20.109(F14): Mod 1 Day 7 FACS analysis: Difference between revisions

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|Yellow Mustard
|Yellow Mustard
|Red Cherries
|Red Cherries
|Pink Bubblegum
|Pink
|-
|-
|II: 2-3:30-pm
|II: 2-3:30-pm
Line 43: Line 43:
| Blue
| Blue
|
|
|-
|}
==Data Analysis==
===Reported data===
To aid class-wide comparisons (and statistics!), please post the requested information in the table below in a timely fashion using three significant digits (ex. 3.72). For samples done in triplicate, please separate the three values with a semi-colon. Once you've completed your statistics, don't get over-excited with the number of significant figures you report! For example, "3.7" will suffice. Please describe the experimental test conditions in the form "0.2 ug D5 and 0.05 ug CUT D3." Be sure to include which enzyme was used to cut D3.
===T/R===
{| border="1"
|Team color
|% R2 Gated Mock
| EGFP
| Δ5
| uncut Δ3
| cut Δ3
| Δ5+uncut Δ3
| Δ5+cut Δ3
| Exp A
|Exp condition
| Exp B
|Exp condition
|-
|Cherries
| 0.01
| 57.6
| 0.69
| 0.47
| 0.08
| 2.27; 2.69; 2.32
| 9.66; 10.14; 10.27 (PmeI)
| 7.63; 6.99; 7.89
| 0.1 ug D5 and 0.1 ug BamHI CUT D3
|5.75; 7.45; 6.29
| 0.1 ug D5 and 0.5 ug BamHI CUT D3
|-
|Tiger
| 0.00
| 46.7
| 0.06
| 0.01
| 0.00
| 2.00; 1.75; 1.33
| 11.7; 11.6; 11.6 (BamHI)
| 5.67; 5.17; 3.74
| 0.1 ug D5 and 0.1 ug Pme CUT D3
| 8.87; 11.4; 8.44
| 0.1 ug D5 and 0.4 ug BamHI CUT D3
|-
|Mustard
|0.00
|54.35
|0.00
|2.21
|1.72
|6.76; 5.89; 5.00
|4.99; 6.39; 7.54 (PmeI)
|10.88; 9.25; 9.50
|0.1 ug D5 and 0.25 ug PmeI CUT D3
|4.10; 3.96; 3.77
|0.1 ug D5 and 0.50 ug PmeI CUT D3
|-
|Green
|0.00
|61.49
|0.20
|0.20
|0.00
|8.12;8.13;7.19
|2.84;2.71;2.54 (PmeI)
|4.68;4.53;4.07
|0.1 ug D5 and 0.5 ug PmeI CUT D3
|7.48;5.58;5.08
|0.1 ug D5 and 0.05 ug PmeI CUT D3
|-
|Blue, best
|0.00
|42.62
|0.05
|0.01
|0.00
|1.88; 2.43; 2.08
|6.19; 8.73; 7.03 (PmeI)
|14.06; 13.65; 16.94
|0.1ug D5 and 0.1ug BamHI CUT D3
|5.43; 5.48; 4.70
|0.1ug D5 and 0.2ug PmeI CUT D3
|-
|Pink
|0.00
|44.01
|0.06
|0.00
|0.00
|1.80; 1.64; 1.88
|7.33; 6.66; 6.67 (BamH1)
|5.69; 6.21; 5.71
|0,1 D5 + 0,1 Pme1 cut D3
|9.56; 11.65; 11.15
|0,1 ug D5 + 0,5 BamH1 Cut D3
|-
|Purple
|0.00
|54.35
|blank
|blank
|0.00
|blank; 2.21; 1.72
|6.76; 5.89; 5.00 (BamH1)
|4.99; 6.39; 7.54
|0.1 ug D5 and 0.1 ug PmeI Cut D3
|10.88; 9.25; 9.50
|0.1 ug D5 and 0.5 ug BamH1 Cut D3
|-
|}
===W/F===
{| border="1"
|Team color
|% R2 Gated Mock
| EGFP
| Δ5
| uncut Δ3
| cut Δ3
| Δ5+uncut Δ3
| Δ5+cut Δ3
| Exp A
|Exp condition
| Exp B
|Exp condition
|-
|Pinkle
|3.19
|41.34
|0.67
|0.04
|0.01
|2.53; 2.54; 2.41
|8.58; 8.65; 8.47 (PmeI)
|6.76; 5.93 (one missing)
|0.1ug D5 and 0.05ug PmeI cut D3
|3.20; 4.06; 4.25
|0.1ug D5 and 0.025ug PmeI cut D3
|-
|Blue
|0.02
|75.9
|0.15
|0.02
|0.00
|3.69; 3.56; 2.60
|25.3; 24.6; 21.7 (BamHI)
|15.2; 16.7; 11.2
|0.1 ug D5 and 0.1 ug PmeI cut D3
|16.3; 15.4; 15.6
|0.1 ug D5 and 1.0 ug BamHI cut D3
|-
|Green
|0.00
|57.05
|0.05
|0.03
|0.02
|2.65; 3.54; 3.04
|7.03; 6.76; 6.93
|20.69; 21.00; 23.06
|0.1 ug of D5 vs. 0.5 ug of BamHI cut D3
|15.68; 17.54; 14.60
|0.1 ug of D5 vs. 1.0 ug of BamHI cut D3
|-
|-
|}
|}

Latest revision as of 21:30, 8 October 2014

Schedule

Sign up for a time to do flow cytometry in the table below. Please arrive at the time you choose and then expect to spend about 45 minutes in TC and another 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.

T/R

Time block Team 1 Team 2 Team 3
I: 1-2:30 pm Yellow Mustard Red Cherries Pink
II: 2-3:30-pm Blue team, best team Green Tiger Team!
III: 3-4:30 pm Purple team

W/F

Time block Team 1 Team 2
I: 1-2:30 pm Pinkle Green
II: 2-3:30-pm Blue


Data Analysis

Reported data

To aid class-wide comparisons (and statistics!), please post the requested information in the table below in a timely fashion using three significant digits (ex. 3.72). For samples done in triplicate, please separate the three values with a semi-colon. Once you've completed your statistics, don't get over-excited with the number of significant figures you report! For example, "3.7" will suffice. Please describe the experimental test conditions in the form "0.2 ug D5 and 0.05 ug CUT D3." Be sure to include which enzyme was used to cut D3.

T/R

Team color % R2 Gated Mock EGFP Δ5 uncut Δ3 cut Δ3 Δ5+uncut Δ3 Δ5+cut Δ3 Exp A Exp condition Exp B Exp condition
Cherries 0.01 57.6 0.69 0.47 0.08 2.27; 2.69; 2.32 9.66; 10.14; 10.27 (PmeI) 7.63; 6.99; 7.89 0.1 ug D5 and 0.1 ug BamHI CUT D3 5.75; 7.45; 6.29 0.1 ug D5 and 0.5 ug BamHI CUT D3
Tiger 0.00 46.7 0.06 0.01 0.00 2.00; 1.75; 1.33 11.7; 11.6; 11.6 (BamHI) 5.67; 5.17; 3.74 0.1 ug D5 and 0.1 ug Pme CUT D3 8.87; 11.4; 8.44 0.1 ug D5 and 0.4 ug BamHI CUT D3
Mustard 0.00 54.35 0.00 2.21 1.72 6.76; 5.89; 5.00 4.99; 6.39; 7.54 (PmeI) 10.88; 9.25; 9.50 0.1 ug D5 and 0.25 ug PmeI CUT D3 4.10; 3.96; 3.77 0.1 ug D5 and 0.50 ug PmeI CUT D3
Green 0.00 61.49 0.20 0.20 0.00 8.12;8.13;7.19 2.84;2.71;2.54 (PmeI) 4.68;4.53;4.07 0.1 ug D5 and 0.5 ug PmeI CUT D3 7.48;5.58;5.08 0.1 ug D5 and 0.05 ug PmeI CUT D3
Blue, best 0.00 42.62 0.05 0.01 0.00 1.88; 2.43; 2.08 6.19; 8.73; 7.03 (PmeI) 14.06; 13.65; 16.94 0.1ug D5 and 0.1ug BamHI CUT D3 5.43; 5.48; 4.70 0.1ug D5 and 0.2ug PmeI CUT D3
Pink 0.00 44.01 0.06 0.00 0.00 1.80; 1.64; 1.88 7.33; 6.66; 6.67 (BamH1) 5.69; 6.21; 5.71 0,1 D5 + 0,1 Pme1 cut D3 9.56; 11.65; 11.15 0,1 ug D5 + 0,5 BamH1 Cut D3
Purple 0.00 54.35 blank blank 0.00 blank; 2.21; 1.72 6.76; 5.89; 5.00 (BamH1) 4.99; 6.39; 7.54 0.1 ug D5 and 0.1 ug PmeI Cut D3 10.88; 9.25; 9.50 0.1 ug D5 and 0.5 ug BamH1 Cut D3

W/F

Team color % R2 Gated Mock EGFP Δ5 uncut Δ3 cut Δ3 Δ5+uncut Δ3 Δ5+cut Δ3 Exp A Exp condition Exp B Exp condition
Pinkle 3.19 41.34 0.67 0.04 0.01 2.53; 2.54; 2.41 8.58; 8.65; 8.47 (PmeI) 6.76; 5.93 (one missing) 0.1ug D5 and 0.05ug PmeI cut D3 3.20; 4.06; 4.25 0.1ug D5 and 0.025ug PmeI cut D3
Blue 0.02 75.9 0.15 0.02 0.00 3.69; 3.56; 2.60 25.3; 24.6; 21.7 (BamHI) 15.2; 16.7; 11.2 0.1 ug D5 and 0.1 ug PmeI cut D3 16.3; 15.4; 15.6 0.1 ug D5 and 1.0 ug BamHI cut D3
Green 0.00 57.05 0.05 0.03 0.02 2.65; 3.54; 3.04 7.03; 6.76; 6.93 20.69; 21.00; 23.06 0.1 ug of D5 vs. 0.5 ug of BamHI cut D3 15.68; 17.54; 14.60 0.1 ug of D5 vs. 1.0 ug of BamHI cut D3
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