Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
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Note: "hypothetical data" just shows made-up numbers similar to what you might expect. | Note: "hypothetical data" just shows made-up numbers similar to what you might expect. | ||
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! Group Colour | |||
! pCX-EGFP (#) | |||
! bkb + ins, no lig (#) | |||
! bkb + lig, no ins (#) | |||
! bkb + ins, lig 1 (#) | |||
! bkb + ins, lig 2 (#) | |||
|- | |||
| Hypothetical Data | |||
| 1000 | |||
| 2 | |||
| 20 | |||
| 100 | |||
| 100 | |||
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Revision as of 13:07, 26 September 2014
Colony Counts
W/F lab
Please put your colony count data in the correct row.
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
Diagnostic Digests
Gel Pattern:
1st Colony: Uncut, RE-1, RE-2, Double Digest
2nd Colony: Uncut, RE-1
1kb Ladder, NEB
2nd Colony: RE-2, Double Digest
pCX-NNX: Uncut, RE-1, RE-2, Double Digest
1kb Ladder, NEB
T/R lab
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
W/F lab
Group Colour | pCX-EGFP (#) | bkb + ins, no lig (#) | bkb + lig, no ins (#) | bkb + ins, lig 1 (#) | bkb + ins, lig 2 (#) |
---|---|---|---|---|---|
Hypothetical Data | 1000 | 2 | 20 | 100 | 100 |