Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
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==Colony Counts== | ==Colony Counts== | ||
===W/F lab== | ===W/F lab=== | ||
Please put your colony count data in the correct row. | |||
Note: "hypothetical data" just shows made-up numbers similar to what you might expect. | |||
{| align="center" border="1" | |||
! Group Colour | |||
! pCX-EGFP (#) | |||
! bkb + ins, no lig (#) | |||
! bkb + lig, no ins (#) | |||
! bkb + ins, lig 1 (#) | |||
! bkb + ins, lig 2 (#) | |||
|- | |||
| Hypothetical Data | |||
| 1000 | |||
| 2 | |||
| 20 | |||
| 100 | |||
| 100 | |||
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==Diagnostic Digests== | ==Diagnostic Digests== | ||
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1kb Ladder, NEB | 1kb Ladder, NEB | ||
===T/R lab== | ===T/R lab=== | ||
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling). | Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling). | ||
Revision as of 12:51, 26 September 2014
Colony Counts
W/F lab
Please put your colony count data in the correct row.
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
Diagnostic Digests
- Gel Pattern:
1st Colony: Uncut, RE-1, RE-2, Double Digest
2nd Colony: Uncut, RE-1
1kb Ladder, NEB
2nd Colony: RE-2, Double Digest
pCX-NNX: Uncut, RE-1, RE-2, Double Digest
1kb Ladder, NEB
T/R lab
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
- Red Group:
- Orange Group:
- Yellow Group:
- Green Group:
- Blue Group:
- Pink Group:
- Purple Group:
==W/F lab==�
Group Colour | pCX-EGFP (#) | bkb + ins, no lig (#) | bkb + lig, no ins (#) | bkb + ins, lig 1 (#) | bkb + ins, lig 2 (#) |
---|---|---|---|---|---|
Hypothetical Data | 1000 | 2 | 20 | 100 | 100 |