Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture

Colony Counts

W/F lab

Please put your colony count data in the correct row.

Note: "hypothetical data" just shows made-up numbers similar to what you might expect.

Diagnostic Digests

Gel Pattern:

1st Colony: Uncut, RE-1, RE-2, Double Digest

2nd Colony: Uncut, RE-1

1kb Ladder, NEB

2nd Colony: RE-2, Double Digest

pCX-NNX: Uncut, RE-1, RE-2, Double Digest

1kb Ladder, NEB

T/R lab

Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).

• Red Group:

RE-1: B ; RE-2: P

• Orange Group:

RE-1: BamHI ; RE-2: PstI

• Yellow Group:

RE-1: B ; RE-2: N

• Green Group:

RE-1: PvuI ; RE-2: EcoRV

• Blue Group:

RE-1: BamHI ; RE-2: SalI

• Pink Group:

RE-1: BamHI ; RE-2: PvuI

• Purple Group:

RE-1: E_1 ; RE-2: E_2

W/F lab

Group Colour	pCX-EGFP (#)	bkb + ins, no lig (#)	bkb + lig, no ins (#)	bkb + ins, lig 1 (#)	bkb + ins, lig 2 (#)
Hypothetical Data	1000	2	20	100	100