Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
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Line 65: | Line 65: | ||
*Red Group: | *Red Group: | ||
[[Image:TR_Red_DD20109_F14.bmp|thumb|350px|right | [[Image:TR_Red_DD20109_F14.bmp|thumb|350px|right|RE-1: B ; RE-2: P]] | ||
RE-1: B ; RE-2: P | |||
*Orange Group: | *Orange Group: | ||
[[Image:TR_Orange_DD20109_F14.bmp|thumb|350px|left | [[Image:TR_Orange_DD20109_F14.bmp|thumb|350px|left|RE-1: BamHI ; RE-2: PstI]] | ||
RE-1: BamHI ; RE-2: PstI | |||
*Yellow Group: | *Yellow Group: | ||
[[Image:TR_Yellow_DD20109_F14.bmp | [[Image:TR_Yellow_DD20109_F14.bmp|thumb|350px|right|RE-1: B ; RE-2: N]] | ||
RE-1: B ; RE-2: N | |||
*Green Group: | *Green Group: | ||
[[Image:TR_Green_DD20109_F14.bmp | [[Image:TR_Green_DD20109_F14.bmp|thumb|350px|left|RE-1: PvuI ; RE-2: EcoRV]] | ||
RE-1: PvuI ; RE-2: EcoRV | |||
*Blue Group: | *Blue Group: |
Revision as of 13:01, 26 September 2014
Colony Counts
W/F lab
Please put your colony count data in the correct row.
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
Diagnostic Digests
Gel Pattern:
1st Colony: Uncut, RE-1, RE-2, Double Digest
2nd Colony: Uncut, RE-1
1kb Ladder, NEB
2nd Colony: RE-2, Double Digest
pCX-NNX: Uncut, RE-1, RE-2, Double Digest
1kb Ladder, NEB
T/R lab
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
- Red Group:
- Orange Group:
- Yellow Group:
- Green Group:
- Blue Group:
- Pink Group:
- Purple Group:
W/F lab
Group Colour | pCX-EGFP (#) | bkb + ins, no lig (#) | bkb + lig, no ins (#) | bkb + ins, lig 1 (#) | bkb + ins, lig 2 (#) |
---|---|---|---|---|---|
Hypothetical Data | 1000 | 2 | 20 | 100 | 100 |