Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
== | ==Colony Counts== | ||
===W/F lab==�= | |||
==Diagnostic Digests== | |||
*'''Gel Pattern:''' | *'''Gel Pattern:''' | ||
| Line 16: | Line 19: | ||
1kb Ladder, NEB | 1kb Ladder, NEB | ||
==T/R lab== | ===T/R lab==�= | ||
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling). | Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling). | ||
| Line 46: | Line 49: | ||
[[Image:TR_Purple_DD20109_F14.bmp]] | [[Image:TR_Purple_DD20109_F14.bmp]] | ||
RE-1: E_1 ; RE-2: E_2 | RE-1: E_1 ; RE-2: E_2 | ||
===W/F lab==�= | |||
Revision as of 12:49, 26 September 2014
Colony Counts
==W/F lab==�
Diagnostic Digests
- Gel Pattern:
1st Colony: Uncut, RE-1, RE-2, Double Digest
2nd Colony: Uncut, RE-1
1kb Ladder, NEB
2nd Colony: RE-2, Double Digest
pCX-NNX: Uncut, RE-1, RE-2, Double Digest
1kb Ladder, NEB
==T/R lab==�
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
- Red Group:
RE-1: B ; RE-2: P
- Orange Group:
RE-1: BamHI ; RE-2: PstI
- Yellow Group:
RE-1: B ; RE-2: N
- Green Group:
RE-1: PvuI ; RE-2: EcoRV
- Blue Group:
RE-1: BamHI ; RE-2: SalI
- Pink Group:
RE-1: BamHI ; RE-2: PvuI
- Purple Group:
RE-1: E_1 ; RE-2: E_2
