Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
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== | ==Colony Counts== | ||
===W/F lab=== | |||
Gel Pattern: | Please put your colony count data in the correct row. | ||
Note: "hypothetical data" just shows made-up numbers similar to what you might expect. | |||
{| border="1" | |||
! Group Colour | |||
! pCX-EGFP (#) | |||
! bkb + ins, no lig (#) | |||
! bkb + lig, no ins (#) | |||
! bkb + ins, lig 1 (#) | |||
! bkb + ins, lig 2 (#) | |||
|- | |||
| Hypothetical Data | |||
| 1000 | |||
| 2 | |||
| 20 | |||
| 100 | |||
| 100 | |||
|- | |||
|Blue | |||
|1600 | |||
|0 | |||
|n/a | |||
|0 | |||
|0 | |||
|- | |||
|Green | |||
|Lawn | |||
|0 | |||
|4 | |||
|13 | |||
|14 | |||
|- | |||
|Pinkle | |||
|Lawn | |||
|2 | |||
|2 | |||
|210 | |||
|305 | |||
|- | |||
|} | |||
<br style="clear:both;"/> | |||
==Diagnostic Digests== | |||
==='''Gel Pattern:'''=== | |||
Colony 1: Uncut, RE-1, RE-2, Double Digest | 1st Colony: Uncut, RE-1, RE-2, Double Digest | ||
2nd Colony: Uncut, RE-1 | |||
1kb Ladder, NEB | |||
2nd Colony: RE-2, Double Digest | |||
pCX-NNX: Uncut, RE-1, RE-2, Double Digest | |||
1kb Ladder, NEB | |||
===T/R lab=== | |||
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling). | |||
[[Image:TR_Red_DD20109_F14.bmp|thumb|350px|right|Red Group: RE-1: B ; RE-2: P]] | |||
[[Image:TR_Orange_DD20109_F14.bmp|thumb|350px|left|Orange Group: RE-1: BamHI ; RE-2: PstI]] | |||
[[Image:TR_Yellow_DD20109_F14.bmp|thumb|350px|right|Yellow Group: RE-1: B ; RE-2: N]] | |||
[[Image:TR_Green_DD20109_F14.bmp|thumb|350px|left|Green Group: RE-1: PvuI ; RE-2: EcoRV]] | |||
[[Image:TR_Blue_DD20109_F14.bmp|thumb|350px|right| Blue Group: RE-1: BamHI ; RE-2: SalI]] | |||
1 | [[Image:TR_Pink_DD20109_F14.bmp|thumb|350px|left|Pink Group: RE-1: BamHI ; RE-2: PvuI]] | ||
= | [[Image:TR_Purple_DD20109_F14.bmp|thumb|350px|left|Purple Group: RE-1: E_1 ; RE-2: E_2]] | ||
<br style="clear:both;"/> | |||
===W/F lab=== | |||
[[Image:WR_Green_low_DD20109_F14.bmp|thumb|350px|left|Green Group, low exposure: RE-1: Bam ; RE-2: Ska]] | |||
[[Image: | |||
[[Image:WR_Green_high_DD20109_F14.bmp|thumb|350px|right|Green Group, high exposure: RE-1: Bam; RE-2: Ska ]] | |||
[[Image: | |||
[[Image:WR_Blue_low_DD20109_F14.bmp|thumb|350px|left|Blue Group, low exposure: RE-1: EcoRV ; RE-2: PvuI ]] | |||
[[Image: | |||
[[Image:WR_Blue_high_DD20109_F14.bmp|thumb|350px|right|Blue Group, high exposure: RE-1: EcoRV ; RE-2: PvuI ]] | |||
[[Image: | |||
[[Image:WR_Purple_DD20109_F14.bmp|thumb|350px|left|Purple Group: RE-1: E1 ; RE-2: E2]] | |||
[[Image: | |||
<br style="clear:both;"/> | |||
Latest revision as of 18:24, 29 September 2014
Colony Counts
W/F lab
Please put your colony count data in the correct row.
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
Group Colour | pCX-EGFP (#) | bkb + ins, no lig (#) | bkb + lig, no ins (#) | bkb + ins, lig 1 (#) | bkb + ins, lig 2 (#) |
---|---|---|---|---|---|
Hypothetical Data | 1000 | 2 | 20 | 100 | 100 |
Blue | 1600 | 0 | n/a | 0 | 0 |
Green | Lawn | 0 | 4 | 13 | 14 |
Pinkle | Lawn | 2 | 2 | 210 | 305 |
Diagnostic Digests
Gel Pattern:
1st Colony: Uncut, RE-1, RE-2, Double Digest
2nd Colony: Uncut, RE-1
1kb Ladder, NEB
2nd Colony: RE-2, Double Digest
pCX-NNX: Uncut, RE-1, RE-2, Double Digest
1kb Ladder, NEB
T/R lab
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
W/F lab