Talk:20.109(F14): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions

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==W/F lab==
==W/F lab==
[[Image:WF_Recovery20109_F14.tiff]]


===Recovery gel===
===Recovery gel===
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[[Image:F14_M1D3_WF-recovery.jpg|550px|thumb|center| W/F Recovery Gel]]
[[Image:F14_M1D3_WF-recovery.jpg|550px|thumb|center| W/F Recovery Gel]]


Team Samples (numbers refer to lanes):
Gel Pattern:
*1-2 xxx
Purified bkb ; 1kb MW marker ; purified PCR
*3-4 xxx
*'''T/R groups: 20 uL of sample was loaded/lane and 0.5 ug of 1kb MW ladder'''
*5 M
 
*6-7 xxx
Team (numbers refer to lanes):
*8-9 additional M?
*1-3 Pink
*4-6 Blue
*7-9 Green


Note: We loaded all of the solution that you left for us, and 20 μL of MW marker was loaded (for a total 1.0 μg).


===Pre-purification gels (for reference/posterity)===
===Pre-purification gels (for reference/posterity)===

Revision as of 11:02, 18 September 2014

T/R lab

Recovery gel

Here is your recovery gel:

Gel Pattern: Purified bkb ; 1kb MW marker ; purified PCR

  • T/R groups: 20 uL of sample was loaded/lane and 0.5 ug of 1kb MW ladder

Top Gel (numbers refer to lanes):

  • 1-3 Red
  • 4-6 Orange
  • 7-9 Yellow
  • 10-12 Green


Bottom Gel (numbers refer to lanes):

  • 1-3 Blue
  • 4-6 Pink
  • 7-9 Purple

W/F lab

Recovery gel

File:F14 M1D3 WF-recovery.jpg
W/F Recovery Gel

Gel Pattern: Purified bkb ; 1kb MW marker ; purified PCR

  • T/R groups: 20 uL of sample was loaded/lane and 0.5 ug of 1kb MW ladder

Team (numbers refer to lanes):

  • 1-3 Pink
  • 4-6 Blue
  • 7-9 Green


Pre-purification gels (for reference/posterity)

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!

Green Group
Blue Group
Pinkle Group