Talk:20.109(F13): Mod 2 Day 6 Analysis & Planning 2: Difference between revisions

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===TR Blots===
===TR Blots===
'''TR Intensities'''
Defaults
*EGFR - 700:5, 800:3
*GAPDH - 700:2, 800:5
*Akt - 700:2, 800: 6
*ERK - 700:1, 800:3
*Pink STAT3 - 700:7, 800:9
*Pink EGFR - 700:7, 800:3
[[Image:Yellow_TR_EGFR.tif|thumb|left|300px|Yellow EGFR]]
[[Image:Yellow_TR_ERK.tif|thumb|left|300px|Yellow ERK]]
[[Image:Purple_TR_EGFR.tif|thumb|left|300px|Purple EGFR]]
[[Image:Purple_TR_ERK.tif|thumb|left|300px|Purple ERK]]
[[Image:White_TR_EGFR.tif|thumb|left|300px|White EGFR]]
[[Image:White_TR_ERK.tif|thumb|left|300px|White ERK]]
[[Image:Green_TR_EGFR.tif|thumb|left|300px|Green EGFR]]
[[Image:Green_TR_pAkt.tif|thumb|left|300px|Green pAkt]]
[[Image:Green_TR_GAPDH.tif|thumb|left|300px|Green GAPDH]]
[[Image:Green_TR_pAkt2.tif|thumb|left|300px|Green pAkt_2]]
[[Image:Green_TR_GAPDH2.tif|thumb|left|300px|Green GAPDH_2]]
[[Image:Green_TR_totalAkt2.tif|thumb|left|300px|Green Total Akt 2]]
[[Image:Orange_TR_EGFR.tif|thumb|left|300px|Orange EGFR]]
[[Image:Orange_pAkt.tif|thumb|left|300px|Orange pAkt]]
[[Image:Orange_TR_GAPDH.tif|thumb|left|300px|Orange GAPDH]]
[[Image:Orange_TR_pAkt2.tif|thumb|left|300px|Orange pAkt_2]]
[[Image:Orange_TR_GAPDH2.tif|thumb|left|300px|Orange GAPDH_2]]
[[Image:Orange_TR_totalAkt2.tif|thumb|left|300px|Orange Total Akt 2]]
[[Image:Pink_TR_EGFR2.tif|thumb|left|300px|Pink EGFR]]
[[Image:Pink_TR_STAT3.tif|thumb|left|300px|Pink STAT3]]
[[Image:Pink_TR_GAPDH.tif|thumb|left|300px|Pink GAPDH]]
[[Image:Blue_TR_EGFR.tif|thumb|left|300px|Blue EGFR]]
[[Image:Blue_TR_STAT3.tif|thumb|left|300px|Blue STAT3]]
[[Image:Blue_TR_GAPDH.tif|thumb|left|300px|Blue GAPDH]]
[[Image:Red_TR_EGFR.tif|thumb|left|300px|Red EGFR]]
[[Image:Red_TR_STAT3.tif|thumb|left|300px|Red STAT3]]
[[Image:Red_TR_GAPDH.tif|thumb|left|300px|Red GAPDH]]
[[Image:Green_EGFR.tif|thumb|left|300px|Green EGFR]]
[[Image:Green_pAkt.tif|thumb|left|300px|Green pAkt]]
[[Image:Green_GAPDH.tif|thumb|left|300px|Green GAPDH]]
[[Image:Plat_EGFR.tif|thumb|left|300px|Plat EGFR]]
[[Image:Plat_pAkt.tif|thumb|left|300px|Plat pAkt]]
[[Image:Plat_GAPDH.tif|thumb|left|300px|Plat GAPDH]]
[[Image:Plat_TR_pAkt2.tif|thumb|left|300px|Plat pAkt_2]]
[[Image:Plat_TR_GAPDH2.tif|thumb|left|300px|Plat GAPDH_2]]
[[Image:Plat_TR_totalAkt2.tif|thumb|left|300px|Plat Total Akt 2]]
<br style="clear:both;"/>


[[Image:Yellow_TR_EGFR.tif|Yellow EGFR]]
===WF Blots===
'''WF Intensities'''
ERK
*White: 2/3
*Pink: 1.5/3
*Green: 2.5/3
STAT3
*Red: 6/7.5
*Blue: 6/8
GAPDH (All Teams)
* 2/5
[[Image:Yellow_WF_EGFR.tif|thumb|center|300px|Yellow EGFR]]
[[Image:Yellow_WF_pAkt.tif|thumb|center|300px|Yellow pAkt]]
[[Image:Yellow_WF_GAPDH.tif|thumb|center|300px|Yellow GAPDH]]
[[Image:Yellow_WF_totalAkt.tif|thumb|center|300px|Yellow total Akt]]
*Note, Yellow blots are in the reverse order.
[[Image:Purple_WF_EGFR.tif|thumb|center|300px|Purple EGFR]]
[[Image:Purple_pAkt.tif|thumb|center|300px|Purple pAkt]]
[[Image:Purple_WF_GAPDH.tif|thumb|center|300px|Purple GAPDH]]
[[Image:Orange_WF_EGFR.tif|thumb|center|300px|Orange EGFR]]
[[Image:Orange_WF_GAPDH.tif|thumb|center|300px|Orange GAPDH]]
[[Image:Red_WF_EGFR.tif|thumb|center|300px|Red EGFR]]
[[Image:Red_WF_STAT3_2.tif|thumb|center|300px|Red STAT3]]
[[Image:Red_WF_GAPDH.tif|thumb|center|300px|Red GAPDH]]
[[Image:Blue_WF_EGFR.tif|thumb|center|300px|Blue EGFR]]
[[Image:Blue_WF_STAT3_2.tif|thumb|center|300px|Blue STAT3]]
[[Image:Blue_WF_GAPDH.tif|thumb|center|300px|Blue GAPDH]]
[[Image:Pink_WF_EGFR.tif|thumb|center|300px|Pink EGFR]]
[[Image:Pink_WF_ERK.tif|thumb|center|300px|Pink ERK]]
[[Image:Green_WF_EGFR.tif|thumb|center|300px|Green EGFR]]
[[Image:Green_WF_ERK.tif|thumb|center|300px|Green ERK]]
[[Image:White_WF_EGFR.tif|thumb|center|300px|White EGFR]]
[[Image:White_WF_Erk.tif|thumb|center|300px|White ERK]]

Latest revision as of 09:17, 6 November 2013

TR Blots

TR Intensities Defaults

  • EGFR - 700:5, 800:3
  • GAPDH - 700:2, 800:5
  • Akt - 700:2, 800: 6
  • ERK - 700:1, 800:3
  • Pink STAT3 - 700:7, 800:9
  • Pink EGFR - 700:7, 800:3
Yellow EGFR
Yellow ERK
Purple EGFR
Purple ERK
White EGFR
White ERK
Green EGFR
Green pAkt
Green GAPDH
Green pAkt_2
Green GAPDH_2
Green Total Akt 2
Orange EGFR
Orange pAkt
Orange GAPDH
Orange pAkt_2
Orange GAPDH_2
Orange Total Akt 2
Pink EGFR
Pink STAT3
Pink GAPDH
Blue EGFR
Blue STAT3
Blue GAPDH
Red EGFR
Red STAT3
Red GAPDH
Green EGFR
Green pAkt
Green GAPDH
Plat EGFR
Plat pAkt
Plat GAPDH
Plat pAkt_2
Plat GAPDH_2
Plat Total Akt 2


WF Blots

WF Intensities ERK

  • White: 2/3
  • Pink: 1.5/3
  • Green: 2.5/3

STAT3

  • Red: 6/7.5
  • Blue: 6/8

GAPDH (All Teams)

  • 2/5
Yellow EGFR
Yellow pAkt
Yellow GAPDH
Yellow total Akt
  • Note, Yellow blots are in the reverse order.
Purple EGFR
Purple pAkt
Purple GAPDH
Orange EGFR
Orange GAPDH
Red EGFR
Red STAT3
Red GAPDH
Blue EGFR
Blue STAT3
Blue GAPDH
Pink EGFR
Pink ERK
Green EGFR
Green ERK
White EGFR
White ERK
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