Talk:20.109(F13): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
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(New page: ===T/R lab=== ====Digest Results==== ===W/F lab=== ====Digest Results==== ====Ligation Results==== Please put your colony count data in the correct row. Note: "hypothetical data" just...) |
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====Digest Results==== | ====Digest Results==== | ||
Note: most of these gels were run using loading dye without RNase. In Agi's misguided attempt to keep your methods section simple, she made the call to stick with the NEB loading dye instead of our home-brew loading dye -- sorry, all! Most of the gels seem straightforward to interpret: just ignore the very thick small bands of RNA, and note that your other DNA bands may be shifted down very slightly and also curved. However, in a couple of cases we may re-run digests/gels, and will keep you posted about it. | |||
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===W/F lab=== | ===W/F lab=== | ||
Revision as of 10:25, 28 September 2013
T/R lab
Digest Results
Note: most of these gels were run using loading dye without RNase. In Agi's misguided attempt to keep your methods section simple, she made the call to stick with the NEB loading dye instead of our home-brew loading dye -- sorry, all! Most of the gels seem straightforward to interpret: just ignore the very thick small bands of RNA, and note that your other DNA bands may be shifted down very slightly and also curved. However, in a couple of cases we may re-run digests/gels, and will keep you posted about it.
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W/F lab
Digest Results
Ligation Results
Please put your colony count data in the correct row.
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
| Group Colour | pCX-EGFP (#) | bkb + ins, no lig (#) | bkb + lig, no ins (#) | bkb + ins, lig 1 (#) | bkb + ins, lig 2 (#) |
|---|---|---|---|---|---|
| Hypothetical Data | 1000 | 2 | 20 | 100 | 100 |
