Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

T/R lab

Here is your recovery gel:

Top Gel (numbers refer to lanes):

• 1-2 Pink
• 3-4 Purple
• 5 MW
• 6-7 White
• 8-9 Blue

Bottom Gel (numbers refer to lanes):

• 1-2 Orange
• 3-4 Yellow
• 5 MW
• 6-7 Green
• 8-9 Platinum
• 10 Red

Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).