Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions
No edit summary |
|||
| (6 intermediate revisions by 3 users not shown) | |||
| Line 5: | Line 5: | ||
==T/R lab== | ==T/R lab== | ||
===Recovery gel=== | |||
Here is your recovery gel: | Here is your recovery gel: | ||
[[Image:TR_F1320109_M1D3_recoverygel.BMP]] | [[Image:TR_F1320109_M1D3_recoverygel.BMP]] | ||
| Line 24: | Line 26: | ||
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg). | Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg). | ||
===Pre-purification gels (for reference/posterity)=== | |||
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page! | |||
[[Image:F13_M1D3_TR-Red.jpg|250px|thumb|right| Red Group]] | |||
[[Image:F13_M1D3_TR-Org.jpg|250px|thumb|left| Orange Group]] | |||
[[Image:F13_M1D3_TR-Ylw.jpg|250px|thumb|center| Yellow Group]] | |||
[[Image:F13_M1D3_TR-Grn.jpg|250px|thumb|right| Green Group]] | |||
[[Image:Mod1Day3AgaroseGel1 copy.jpg|250px|thumb|left| Blue Group]] | |||
[[Image:F13_M1D3_TR-Pnk.jpg|250px|thumb|center| Pink Group]] | |||
[[Image:F13_M1D3_TR-Prp.jpg|250px|thumb|right| Purple Group]] | |||
[[Image:F13_M1D3_TR-Sil.jpg|250px|thumb|left| Silver Group]] | |||
[[Image:F13_M1D3_TR-Whi.jpg|250px|thumb|center| White Group]] | |||
<br style="clear:both;"/> | |||
==W/F lab== | ==W/F lab== | ||
===Recovery gel=== | ===Recovery gel=== | ||
[[Image:F13_M1D3_WF-recovery.jpg|550px|thumb|center| W/F Recovery Gel]] | |||
Top Gel (numbers refer to lanes): | |||
*1-2 Red | |||
*3-4 Orange | |||
*5 MW | |||
*6-7 Yellow | |||
*8-9 Green | |||
Bottom Gel (numbers refer to lanes): | |||
*1-2 Blue | |||
*3-4 Pink | |||
*5 MW | |||
*6-7 Purple | |||
*8-9 White | |||
Note: We loaded all of the solution that you left for us, and 20 μL of MW marker was loaded (for a total 1.0 μg). | |||
===Pre-purification gels (for reference/posterity)=== | ===Pre-purification gels (for reference/posterity)=== | ||
Latest revision as of 08:34, 24 September 2013
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
T/R lab
Recovery gel
Here is your recovery gel:
Top Gel (numbers refer to lanes):
- 1-2 Pink
- 3-4 Purple
- 5 MW
- 6-7 White
- 8-9 Blue
Bottom Gel (numbers refer to lanes):
- 1-2 Orange
- 3-4 Yellow
- 5 MW
- 6-7 Green
- 8-9 Platinum
- 10 Red
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).
Pre-purification gels (for reference/posterity)
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!
W/F lab
Recovery gel
Top Gel (numbers refer to lanes):
- 1-2 Red
- 3-4 Orange
- 5 MW
- 6-7 Yellow
- 8-9 Green
Bottom Gel (numbers refer to lanes):
- 1-2 Blue
- 3-4 Pink
- 5 MW
- 6-7 Purple
- 8-9 White
Note: We loaded all of the solution that you left for us, and 20 μL of MW marker was loaded (for a total 1.0 μg).
Pre-purification gels (for reference/posterity)
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!
