Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis

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Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

T/R lab

Recovery gel

Here is your recovery gel:

Top Gel (numbers refer to lanes):

1-2 Pink
3-4 Purple
5 MW
6-7 White
8-9 Blue

Bottom Gel (numbers refer to lanes):

1-2 Orange
3-4 Yellow
5 MW
6-7 Green
8-9 Platinum
10 Red

Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).

Pre-purification gels (for reference/posterity)

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!

W/F lab

Recovery gel

Top Gel (numbers refer to lanes):

1-2 Red
3-4 Orange
5 MW
6-7 Yellow
8-9 Green

Bottom Gel (numbers refer to lanes):

1-2 Blue
3-4 Pink
5 MW
6-7 Purple
8-9 White

Note: We loaded all of the solution that you left for us, and 20 μL of MW marker was loaded (for a total 1.0 μg).

Pre-purification gels (for reference/posterity)

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!