Talk:20.109(F12): Mod 1 Day 7 FACS analysis

Sign up for time on the FACS. Please arrive at the time you choose and then expect to spend 45 minutes in TC and 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.

T/R FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.

team color	FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM)	mock data	EGFP data	delta 5 data	delta 3 data	delta5:delta3 (1:1)	delta5:delta3(1:2)	delta5:delta3(1:10)	extra experiment (specify below)
Red	block I = 12:30-2PM	0.02	55.81	0.00	0.00	1.46	2.08	1.45	1.40 (1:1 ratio with 10' incubation time)
Orange	block II = 1:30-3PM	0.00	58.46	0.00	0.01	2.05	2.11	1.96	1.42 (2:1 ratio)
Yellow	block I = 12:30-2PM
Green	Block III - 2:30-4pm	0.00	50.75	0.00	0.04	1.65	2.69	1.06	1:1 ratio with 10min incubation time - 1.23
Blue	Block II - 1:30-3pm
Pink	block I = 12:30-2PM	0.00	56.14	0.00	0.00	1.91	2.51	.36	2.07 (10:1 ratio)
Purple	Block II = 1:30-3PM	0.00	50.36	0.00	0.04	2.01	2.65	1.27	2.91 at 2:1 ratio (5:3)

W/F FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.

team color	FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM)	mock data	EGFP data	delta 5 data	delta 3 data	delta5:delta3 (1:1)	delta5:delta3 (1:2)	delta5:delta3 (1:10)	extra experiment (specify below)
Purple	Block I = 12:30-2:00pm	0	64.96	0	0.01	1.58	3.46	1.78	1:5 ratio - 3.21
Orange
Yellow
Green
Blue	Block II= 1:30-3pm	0	24.39	0	0	0.65	0.84	0.36 (used 1:5 ratio)	2:1 ratio: 0.19
Pink	Block II = 1:30-3PM	0	62.84	0	0.01	1.30	2.02	0.64	1:1 ratio with higher concentration (0.15ug each): 1.61%