Talk:20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions

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=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
[[Image:TR Row1.jpg]]
[[Image:TR Row1.jpg]]
Row 1 legend is
Row 1 legend is
#Red backbone
#Red backbone
Line 44: Line 43:
#Green backbone
#Green backbone
#Green fragment
#Green fragment
[[Image:TR Row2.jpg]]
Row 2 legend:
#Blue backbone
#Blue fragment
#1 kb ladder
#Pink backbone
#Pink fragment
#1 kb ladder
#Purple backbone


=W/F Lab=
=W/F Lab=

Revision as of 15:20, 18 September 2012

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

You can use these in your assignments if you so choose.



T/R lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

Row 1 legend is

  1. Red backbone
  2. Red fragment
  3. 1 kb ladder
  4. Orange backbone
  5. Orange fragment
  6. Yellow backbone
  7. Yellow fragment
  8. 1 kb ladder
  9. Green backbone
  10. Green fragment

Row 2 legend:

  1. Blue backbone
  2. Blue fragment
  3. 1 kb ladder
  4. Pink backbone
  5. Pink fragment
  6. 1 kb ladder
  7. Purple backbone

W/F Lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

You can upload the pre-purification gels for reference here