Talk:20.109(F11): Mod 2 Day 6 Readouts of DNA, Protein
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Mutant Summary Data
Please summarize your findings in the appropriate table below. Include your first name and last initial so people who want to know more about your data can contact you. For the purposes of this table, just say that B-gal decreased/increased in the dark/light (relative to wild type), rather than giving exact Miller units.
Group Color | Group Names | Mutant 1 sequence change(s) | Mutant 1 B-gal summary results | Mutant 2 sequence change(s) | Mutant 2 B-gal summary results |
---|---|---|---|---|---|
T/R_Red | Shelley & Laura | A -> T | lower than kinase dead in light and dark | no mutation | similar to WT in light, lower beta gal in dark |
T/R_Orange | |||||
T/R_Yellow | Cara, Queenie, Hannah | Data inconclusive | decrease in light & dark | Ala --> Glu | very low B-gal activity in light & dark |
T/R_Green | Jon, Michael | Unknown | Decreased B-gal activity in light, better contrast | Unknown | Low B-gal activity in both light & dark |
T/R_Blue | E. Brown, R. Park | B-gal decrease in light and dark | B-gal decrease in light and dark | ||
T/R_Pink | Colin Beckwitt, Jamal Elkhader | No Mutation | B-gal decrease in light and dark (dark and light activity identical) | A553->K553(GCG->AAG), I566->V566(ATT->GTT) | B-gal decrease in light and dark (dark activity higher than light) |
T/R_Purple |
Group Color | Group Names | Mutant 1 sequence change(s) | Mutant 1 B-gal summary results | Mutant 2 sequence change(s) | Mutant 2 B-gal summary results |
---|---|---|---|---|---|
W/F_Red | Brook W, Edgar M | frameshift via deletions | Similar B-gal activity under both conditions (higher activity than light wild type) | frameshift via insertion | Similar B-gal activity under both conditions (higher activity than light wild type) |
W/F_Orange | Mariana D, Divya C | Ala --> Val (GCG --> GTG) | B-gal decreased both light and dark (dark and light activity identical) | No mutation | B-gal decreased in light |
W/F_Yellow | Robin Y, Brian D | A -> T | decreased in both light and dark (no difference) | A -> I | decreased in both light and dark (no difference) |
W/F_Green | Paul M. Cuong N. | B-gal decrease in both light and dark. | B-gal decrease in dark. | ||
W/F_Blue | Yimin C, Dan G | Ala-->Ser | B-gal decreased in both dark/light | Ala-->Asn | B-gal decreased in both dark/light |
W/F_Pink/Purple | Luis J, Dorma F, Michelle F, | A553K | Bacterial photograph lighter than WT (in the dark). | A553K and I566V | Bacterial photograph lighter than WT (in the dark). |