Talk:20.109(F10): Mod 1 Day 8 FACS analysis: Difference between revisions

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Line 81: Line 81:
*Sample 5: Δ5+Δ3(uncut)
*Sample 5: Δ5+Δ3(uncut)
*Sample 6: EGFP
*Sample 6: EGFP
[[Image:Purple 10-7-10.pdf]]
1.Mock
2.delta 5
3.delta 3
4.delta 5 + cut delta 3
5.delta 5 + cut delta 3 (delta 3 irradiated with UV for 30s)
6. EGFP

Latest revision as of 08:49, 9 October 2010

Sign up for time on the BioAnalyzer. Please arrive at the time you choose and then expect to spend one hour in TC and one hour at the BioAnalyzer. TWO or THREE teams should sign up for each time slot.

T/R BioA schedule

team color BioA time block (I = 12:30-2:30PM, II = 1:30-3:30PM, III = 2:30-4:30PM, as needed IV = 3:30-5PM)
Red 1:30-3:30
Orange 12:30-2:30
Yellow 1:30-3:30
Green II 1:30-3:30PM
Blue I:12:30-2:30
Pink BioA time block I (12:30-2:30)
Purple 2:30-4:30
Gray I (12:30)

T/R BioA Data

File:Green 10-7-10.pdf

  • Sample 1: Mock + EtBR
  • Sample 2: Δ3(cut)
  • Sample 3: Δ5
  • Sample 4: Δ5+Δ3(cut)
  • Sample 5: Δ5+Δ3 + EtBr
  • Sample 6: EGFP


File:Blue 07Oct10.pdf
File:Pink 07Oct10.pdf

  • Sample 1: Mock
  • Sample 2: Δ3(cut)
  • Sample 3: Δ5
  • Sample 4: Δ5+Δ3(cut)
  • Sample 5: Δ5+Δ3(cut, 2 minute UV exposure)
  • Sample 6: EGFP


File:Yellow 07Oct10.pdf


  • Sample 1: Mock
  • Sample 2: Δ5
  • Sample 3: Δ3(cut)
  • Sample 4: Δ5+Δ3(cut)
  • Sample 5: Δ5+Δ3(cut, 2 minute UV exposure)
  • Sample 6: EGFP


File:Silver 07Oct10.pdf

  • Sample 1: Mock
  • Sample 2: Δ3(cut)
  • Sample 3: Δ5
  • Sample 4: Δ5+Δ3(cut)
  • Sample 5: Δ5+Δ3(uncut)
  • Sample 6: EGFP


File:Orange 10-7-10.pdf

File:Red 10-7-10.pdf

  • Sample 1: Mock
  • Sample 2: Δ3(cut)
  • Sample 3: Δ5
  • Sample 4: Δ5+Δ3(cut)
  • Sample 5: Δ5+Δ3(uncut)
  • Sample 6: EGFP

File:Purple 10-7-10.pdf 1.Mock 2.delta 5 3.delta 3 4.delta 5 + cut delta 3 5.delta 5 + cut delta 3 (delta 3 irradiated with UV for 30s) 6. EGFP