Talk:20.109(F07): Luciferase assays and RNA prep

Luciferase Assay Data

Refer to table from first day of module to know what part of the Renilla luciferase gene was targeted by each group.
Please list values for each of your duplicate samples, separated by a comma.
Please calculate out any dilutions that you may have performed on your lysates so that the values listed here reflect the measurements for undiluted samples.
If there were any unusual things that are worth noting about your experiment, please note them below the table for your day of lab and "sign" your comment with your team color.

T/R lab

Sample	Green	Purple	Red	Blue	Pink
No DNA				.012, .0515
plasmid only				23.49, 682.95
siRNA only				.006, .0615
plasmid+siRNA (validated)				19.56, 61.78
plasmid+siRNA (scrambled)				20.67, 603.95
plasmid+siRNA (experimental)				17.3, 182.4

! Yellow |- | No DNA | | | | 11.47, 9.00 | | |- | plasmid only | | | | 29.50, 30.04 | | |- | siRNA only | | | | 4.40, 10.40 | | |- | plasmid+siRNA (validated) | | | | 3.86, 4.05 | | |- | plasmid+siRNA (scrambled) | | | | 32.29, 31.25 | | |- | plasmid+siRNA (experimental) | | | | 15.58, 17.75 | | |}

W/F lab

Sample	Green	Purple	Red	Blue	Pink	Yellow
No DNA	--. 0.042
plasmid only	479.6, 206.2
siRNA only	0.055, 0.058
plasmid+siRNA (validated)	210.6, 130.5
plasmid+siRNA (scrambled)	1749, 1478
plasmid+siRNA (experimental)	113, 107.1