Talk:20.109(F07):Start-up genome engineering: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 106: | Line 106: | ||
| TRgreen_top | | TRgreen_top | ||
| NO | | NO | ||
| GAT CGG TAC CAC GTC | | 5' GAT CGG TAC CAC GTC 3' | ||
| [[Media:Base_insert_for_BamHI.doc| Green Insert Design]] | | [[Media:Base_insert_for_BamHI.doc| Green Insert Design]] | ||
| KpnI BmgI | | KpnI BmgI | ||
| Line 112: | Line 112: | ||
| TRgreen_btm | | TRgreen_btm | ||
| NO | | NO | ||
| CC | | 5' G ATC GAC GTG GTA CC 3' | ||
| | | | ||
| | | | ||
Revision as of 23:15, 11 September 2007
| oligo name | oligo number | oligo sequence | primer record | enzymes added with insert | |
|---|---|---|---|---|---|
| TeacherOligo_top | NO196 | 5' GAT CTG CGC ACG CGT | teacher sample oligo record | FspI and MluI | |
| TeacherOligo_btm | NO197 | 5'G,ATC,ACG,CGT,GCG,CA | |||
| M13KO7seq_2101_fwd | NO198 | 5'- CAA GGC ACT GAC CCC GTT AAA ACT TAT TAC -3' | |||
| M13KO7seq_2151_fwd | NO199 | 5'- AAA AGC CAT GTA TGA CGC TTA CTG GAA CGG -3' |
T/R lab
| oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
|---|---|---|---|---|
| TRpink_top | NO | 5’ GAT CTC GAC GGC GCC 3’ | Oligo design record | Sal I |
| TRpink_btm | NO | 5’ G ATC GGC GCC GTC GA 3’ | Oligo design record | Sfo I |
| TRyellow_top | NO | 5' GAT CTA GAT ATC 3' | Yellow Insert Design | Xba I, Eco RV |
| TRyellow_btm | NO | 5' G ATC GAT ATC TA 3' | Yellow Insert Design | |
| TRblue_top | NO | 5' GAT CAG ATC TTA GGC GCC CGA 3' | Blue Insert Design | BglII |
| TRblue_btm | NO | 5' G ATC TGC GGC GCC TAA GAT CT 3' | Blue Insert Design | SfoI |
| TRpurple_top | NO | 5' GAT CTG ATC AGT ACT 3' | Purple Insert Design | BclI, ScaI |
| TRpurple_btm | NO | 5' GAT CAG TAC TGA TCA 3' | ||
| TRred_top | NO | 5' GAT CTT AAG GAC GTC | Red Insert Design | AflII ZraI |
| TRred_btm | NO | 5' G ATC GAC GTC CTT AA | ||
| TRgreen_top | NO | 5' GAT CGG TAC CAC GTC 3' | Green Insert Design | KpnI BmgI |
| TRgreen_btm | NO | 5' G ATC GAC GTG GTA CC 3' |
W/F lab
| oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
|---|---|---|---|---|
| WFpink_top | NO | |||
| WFpink_btm | NO | |||
| WFyellow_top | NO | |||
| WFyellow_btm | NO | |||
| WFblue_top | NO | |||
| WFblue_btm | NO | |||
| WFpurple_top | NO | |||
| WFpurple_btm | NO | |||
| WFred_top | NO | |||
| WFred_btm | NO | |||
| WFgreen_top | NO | |||
| WFgreen_btm | NO |
[[Media:Media:Example.oggMedia:Example.ogg]]
