Talk:20.109(F07):Start-up genome engineering: Difference between revisions
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Line 108: | Line 108: | ||
| NO | | NO | ||
| GAT CGG TAC CAC GTC | | GAT CGG TAC CAC GTC | ||
| | | [[Media:Base_insert_for_BamHI.doc| Green Insert Design]] | ||
| KpnI BmgI | | KpnI BmgI | ||
|- | |- |
Revision as of 14:12, 11 September 2007
oligo name | oligo number | oligo sequence | primer record | enzymes added with insert | |
---|---|---|---|---|---|
TeacherOligo_top | NO196 | 5' GAT CTG CGC ACG CGT | teacher sample oligo record | FspI and MluI | |
TeacherOligo_btm | NO197 | 5'G,ATC,ACG,CGT,GCG,CA | |||
M13KO7seq_2101_fwd | NO198 | 5'- CAA GGC ACT GAC CCC GTT AAA ACT TAT TAC -3' | |||
M13KO7seq_2151_fwd | NO199 | 5'- AAA AGC CAT GTA TGA CGC TTA CTG GAA CGG -3' |
T/R lab
oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
---|---|---|---|---|
TRpink_top | NO | 5’ GAT CTC GAC GGC GCC 3’ | Oligo design record | Sal I |
TRpink_btm | NO | 5’ G ATC GGC GCC GTC GA 3’ | Oligo design record | Sfo I |
TRyellow_top | NO | 5' GAT CTA GAT ATC 3' | Yellow Insert Design | Xba I, Eco RV |
TRyellow_btm | NO | 5' G ATC GAT ATC TA 3' | Yellow Insert Design | |
TRblue_top | NO | |||
TRblue_btm | NO | |||
TRpurple_top | NO | |||
TRpurple_btm | NO | |||
TRred_top | NO | |||
TRred_btm | NO | |||
TRgreen_top | NO | GAT CGG TAC CAC GTC | Green Insert Design | KpnI BmgI |
TRgreen_btm | NO | CC ATG GTG CAG CTA G |
W/F lab
oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
---|---|---|---|---|
WFpink_top | NO | |||
WFpink_btm | NO | |||
WFyellow_top | NO | |||
WFyellow_btm | NO | |||
WFblue_top | NO | |||
WFblue_btm | NO | |||
WFpurple_top | NO | |||
WFpurple_btm | NO | |||
WFred_top | NO | |||
WFred_btm | NO | |||
WFgreen_top | NO | |||
WFgreen_btm | NO |
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