T broth

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T-Broth (not to be confused with Terrific broth)


  1. 10 g Bacto-tryptone
  2. 5 g NaCl


  1. Mix dry ingredients and add distilled water up to 1 Liter
  2. Pour into 2 L flask (or greater)
  3. Autoclave (liquid cycle)
    • 250°F, 22psi, 30 minutes

Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0. We usually obtain this from the kitchen.


Don't remember.

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