T broth

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Notes: We do not pH medium when we make it on the fly.  However, if it is really important, pH the medium to 7.0.  We usually obtain this from the kitchen.   
Notes: We do not pH medium when we make it on the fly.  However, if it is really important, pH the medium to 7.0.  We usually obtain this from the kitchen.   
==Source==
==Source==
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Don't remember.
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Studier, FW Virology '''39''':562 (1969) Link: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4902069&query_hl=1 Pubmed]

Revision as of 13:40, 12 September 2005

Contents

Summary

T-Broth (not to be confused with Terrific broth)

Ingredients

  1. 10 g Bacto-tryptone
  2. 5 g NaCl

Protocol

  1. Mix dry ingredients and add distilled water up to 1 Liter
  2. Pour into 2 L flask (or greater)
  3. Autoclave (liquid cycle)
    • 250°F, 22psi, 30 minutes

Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0. We usually obtain this from the kitchen.

Source

Studier, FW Virology 39:562 (1969) Link: Pubmed

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