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TSS - Revision history
2013-05-24T15:45:44Z
Revision history for this page on the wiki
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http://openwetware.org/index.php?title=TSS&diff=688934&oldid=prev
Yanglong Zhu: /* Storage */
2013-04-04T18:08:01Z
<p><span class="autocomment">Storage</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:08, 4 April 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C. <del class="diffchange diffchange-inline">CT Chung paper recommends long storage at -80˚C, while people have been using a wide range of temperatures from -20˚C to -140˚C for long term storage.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Escherichia coli]] [[Category:Material]] [[Category:Buffers]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Escherichia coli]] [[Category:Material]] [[Category:Buffers]]</div></td></tr>
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Yanglong Zhu
http://openwetware.org/index.php?title=TSS&diff=688930&oldid=prev
Yanglong Zhu: /* Storage */
2013-04-04T18:02:53Z
<p><span class="autocomment">Storage</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:02, 4 April 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C<ins class="diffchange diffchange-inline">. CT Chung paper recommends long storage at -80˚C, while people have been using a wide range of temperatures from -20˚C to -140˚C for long term storage</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Escherichia coli]] [[Category:Material]] [[Category:Buffers]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Escherichia coli]] [[Category:Material]] [[Category:Buffers]]</div></td></tr>
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Yanglong Zhu
http://openwetware.org/index.php?title=TSS&diff=688926&oldid=prev
Yanglong Zhu: /* Notes */
2013-04-04T17:58:33Z
<p><span class="autocomment">Notes</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:58, 4 April 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Note 1: pH from original Chung CT paper calls for pH to be acidic (pH=6.5)... be sure to pH solution before sterilization</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Note 1<ins class="diffchange diffchange-inline">: PEG 3350 can be used instead of PEG 8000. According to the original CT Chung paper, PEG 3350 produces better efficiency, and other sized PEGs can be used as well albeit with a slight loss in efficiency.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Note <del class="diffchange diffchange-inline">2</del>: If using non-chemically resistant filters (e.g., cellulose nitrate), add DMSO after sterilization. DMSO should be sterile in and of itself, so it may be prudent it add it afterwards if you are unsure about the compatibility of your filters. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Note 2</ins>: pH from original Chung CT paper calls for pH to be acidic (pH=6.5)... be sure to pH solution before sterilization<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Note <ins class="diffchange diffchange-inline">3</ins>: If using non-chemically resistant filters (e.g., cellulose nitrate), add DMSO after sterilization. DMSO should be sterile in and of itself, so it may be prudent it add it afterwards if you are unsure about the compatibility of your filters.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td></tr>
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Yanglong Zhu
http://openwetware.org/index.php?title=TSS&diff=317657&oldid=prev
Skosuri at 15:51, 22 June 2009
2009-06-22T15:51:25Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:51, 22 June 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Filter sterilize (0.22 &mu;m filter)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Filter sterilize (0.22 &mu;m filter)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Notes==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Note 1: pH from original Chung CT paper calls for pH to be acidic (pH=6.5)... be sure to pH solution before sterilization</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Note 2: If using non-chemically resistant filters (e.g., cellulose nitrate), add DMSO after sterilization. DMSO should be sterile in and of itself, so it may be prudent it add it afterwards if you are unsure about the compatibility of your filters. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td></tr>
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Skosuri
http://openwetware.org/index.php?title=TSS&diff=190497&oldid=prev
Torsten Waldminghaus: added tag
2008-03-11T21:37:18Z
<p>added tag</p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 21:37, 11 March 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category:Escherichia coli]] [[Category:Material]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category:Escherichia coli]] [[Category:Material<ins class="diffchange diffchange-inline">]] [[Category:Buffers</ins>]]</div></td></tr>
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Torsten Waldminghaus
http://openwetware.org/index.php?title=TSS&diff=111022&oldid=prev
Reshma P. Shetty at 17:35, 20 April 2007
2007-04-20T17:35:51Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:35, 20 April 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Escherichia coli]] [[Category:Material]]</ins></div></td></tr>
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Reshma P. Shetty
http://openwetware.org/index.php?title=TSS&diff=90441&oldid=prev
Reshma P. Shetty at 22:46, 5 December 2006
2006-12-05T22:46:58Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 22:46, 5 December 2006</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Category:E.Coli Protocol]]</del></div></td><td colspan="2"> </td></tr>
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Reshma P. Shetty
http://openwetware.org/index.php?title=TSS&diff=17553&oldid=prev
Barry Canton at 17:36, 16 January 2006
2006-01-16T17:36:09Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:36, 16 January 2006</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Storage==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Store at 4˚C or -20˚C.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:E.Coli Protocol]]</ins></div></td></tr>
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Barry Canton
http://openwetware.org/index.php?title=TSS&diff=17545&oldid=prev
Barry Canton at 17:14, 16 January 2006
2006-01-16T17:14:28Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:14, 16 January 2006</td>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Uses==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">TSS buffer is used to make [[Preparing chemically competent cells|chemically competent cells]].</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Preparation==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To make 50 mL:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To make 50 mL:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*5g PEG 8000</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*5g PEG 8000</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Add LB to 50 mL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Add LB to 50 mL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Filter sterilize (0.22 &mu;m filter) <del class="diffchange diffchange-inline">and store </del>at 4˚C or -20˚C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Filter sterilize (0.22 &mu;m filter)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==Storage==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Store </ins>at 4˚C or -20˚C.</div></td></tr>
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Barry Canton
http://openwetware.org/index.php?title=TSS&diff=17544&oldid=prev
Barry Canton at 17:13, 16 January 2006
2006-01-16T17:13:28Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:13, 16 January 2006</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*1.5 mL 1M MgCl<sub>2</sub> (or 0.30g MgCl<sub>2</sub>*6H<sub>2</sub>0)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*1.5 mL 1M MgCl<sub>2</sub> (or 0.30g MgCl<sub>2</sub>*6H<sub>2</sub>0)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*2.5 mL DMSO</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*2.5 mL DMSO</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*LB to 50 mL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">Add </ins>LB to 50 mL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Store </del>at <del class="diffchange diffchange-inline">4C</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Filter sterilize (0.22 &mu;m filter) and store </ins>at <ins class="diffchange diffchange-inline">4˚C or -20˚C.</ins></div></td></tr>
<!-- diff generator: internal 2013-05-24 15:45:44 -->
</table>
Barry Canton