TSS
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Store at 4˚C or -20˚C. | Store at 4˚C or -20˚C. | ||
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Revision as of 17:37, 11 March 2008
Uses
TSS buffer is used to make chemically competent cells.
Preparation
To make 50 mL:
- 5g PEG 8000
- 1.5 mL 1M MgCl2 (or 0.30g MgCl2*6H20)
- 2.5 mL DMSO
- Add LB to 50 mL
Filter sterilize (0.22 μm filter)
Storage
Store at 4˚C or -20˚C.
