TSS: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Barry Canton (talk | contribs) No edit summary |
Barry Canton (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
==Uses== | |||
TSS buffer is used to make [[Preparing chemically competent cells|chemically competent cells]]. | |||
==Preparation== | |||
To make 50 mL: | To make 50 mL: | ||
*5g PEG 8000 | *5g PEG 8000 | ||
| Line 5: | Line 10: | ||
*Add LB to 50 mL | *Add LB to 50 mL | ||
Filter sterilize (0.22 μm filter) | Filter sterilize (0.22 μm filter) | ||
==Storage== | |||
Store at 4˚C or -20˚C. | |||
Revision as of 10:14, 16 January 2006
Uses
TSS buffer is used to make chemically competent cells.
Preparation
To make 50 mL:
- 5g PEG 8000
- 1.5 mL 1M MgCl2 (or 0.30g MgCl2*6H20)
- 2.5 mL DMSO
- Add LB to 50 mL
Filter sterilize (0.22 μm filter)
Storage
Store at 4˚C or -20˚C.
