TSS

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*1.5 mL 1M MgCl<sub>2</sub> (or 0.30g MgCl<sub>2</sub>*6H<sub>2</sub>0)
*1.5 mL 1M MgCl<sub>2</sub> (or 0.30g MgCl<sub>2</sub>*6H<sub>2</sub>0)
*2.5 mL DMSO
*2.5 mL DMSO
-
*LB to 50 mL
+
*Add LB to 50 mL
-
Store at 4C
+
Filter sterilize (0.22 &mu;m filter) and store at 4˚C or -20˚C.

Revision as of 13:13, 16 January 2006

To make 50 mL:

  • 5g PEG 8000
  • 1.5 mL 1M MgCl2 (or 0.30g MgCl2*6H20)
  • 2.5 mL DMSO
  • Add LB to 50 mL

Filter sterilize (0.22 μm filter) and store at 4˚C or -20˚C.

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