TE antigen retrieval and immunostaining: Difference between revisions
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This 2-part protocol describes antigen retrieval with Tris EDTA buffer followed by immunostaining. Antigen retrieval can be helpful for aldehyde-fixed, paraffin-embedded samples that don't give sufficient signal with a standard immunostaining protocol. Several [[antigen retrieval]] methods exist but, unfortunately, you need to determine empirically which one is most suited to your antibody. | This 2-part protocol describes antigen retrieval with Tris EDTA buffer followed by immunostaining. Antigen retrieval can be helpful for aldehyde-fixed, paraffin-embedded samples that don't give sufficient signal with a standard immunostaining protocol. Several [[antigen retrieval]] methods exist but, unfortunately, you need to determine empirically which one is most suited to your antibody. | ||
== Deparaffinisation == | |||
== Tris EDTA heat-based antigen retrieval == | == Tris EDTA heat-based antigen retrieval == | ||
=== | === Principle === | ||
=== Material === | === Material === | ||
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=== Comments === | === Comments === | ||
== Immunostaining == | |||
Revision as of 05:48, 2 November 2009
This 2-part protocol describes antigen retrieval with Tris EDTA buffer followed by immunostaining. Antigen retrieval can be helpful for aldehyde-fixed, paraffin-embedded samples that don't give sufficient signal with a standard immunostaining protocol. Several antigen retrieval methods exist but, unfortunately, you need to determine empirically which one is most suited to your antibody.
