TE antigen retrieval and immunostaining

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This 2-part protocol describes antigen retrieval with Tris EDTA buffer followed by immunostaining. Antigen retrieval can be helpful for aldehyde-fixed, paraffin-embedded samples that don't give sufficient signal with a standard immunostaining protocol. Several [[antigen retrieval]] methods exist but, unfortunately, you need to determine empirically which one is most suited to your antibody.
This 2-part protocol describes antigen retrieval with Tris EDTA buffer followed by immunostaining. Antigen retrieval can be helpful for aldehyde-fixed, paraffin-embedded samples that don't give sufficient signal with a standard immunostaining protocol. Several [[antigen retrieval]] methods exist but, unfortunately, you need to determine empirically which one is most suited to your antibody.
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== Deparaffinisation ==
== Tris EDTA heat-based antigen retrieval ==
== Tris EDTA heat-based antigen retrieval ==
   
   
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=== Prinicple ===
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=== Principle ===
=== Material ===
=== Material ===
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=== Comments ===
=== Comments ===
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== Immunostaining ==

Revision as of 07:48, 2 November 2009

This 2-part protocol describes antigen retrieval with Tris EDTA buffer followed by immunostaining. Antigen retrieval can be helpful for aldehyde-fixed, paraffin-embedded samples that don't give sufficient signal with a standard immunostaining protocol. Several antigen retrieval methods exist but, unfortunately, you need to determine empirically which one is most suited to your antibody.

Contents

Deparaffinisation

Tris EDTA heat-based antigen retrieval

Principle

Material

Steps

Comments

Immunostaining

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