TE - Revision history

Etienne Robillard at 14:56, 24 January 2013

2013-01-24T14:56:26Z

←Older revision		Revision as of 14:56, 24 January 2013
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		+	TE is a shorter name for "Tris-EDTA". For the rest of the story, see [[TAE]], [[Tris]], and [[TBE]].
		+
	==Purpose==		==Purpose==
-	TE buffer is often used to store DNA and RNA.	+	* TE buffer is often used to store DNA and RNA.
	* [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.		* [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.
	* [[Tris]] is a buffering agent to keep the solution at a defined pH.		* [[Tris]] is a buffering agent to keep the solution at a defined pH.
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	* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8018 10x TE buffer at Cold Spring Harbor Protocols]		* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8018 10x TE buffer at Cold Spring Harbor Protocols]
	* [http://www.cytographica.com/lab/solutions/TE.htm 10x and 1x TE at cytographica]		* [http://www.cytographica.com/lab/solutions/TE.htm 10x and 1x TE at cytographica]
-		+	* http://en.wikipedia.org/wiki/TE_buffer

	[[Category:Material]]		[[Category:Material]]
	[[Category:Buffers]]		[[Category:Buffers]]

Jakob Suckale at 19:01, 2 November 2009

2009-11-02T19:01:13Z

←Older revision		Revision as of 19:01, 2 November 2009
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	TE buffer is often used to store DNA and RNA.		TE buffer is often used to store DNA and RNA.
	* [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.		* [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.
-	* Tris is a buffering agent to keep the solution at a defined pH.	+	* [[Tris]] is a buffering agent to keep the solution at a defined pH.

	==Recipe 10x TE ==		==Recipe 10x TE ==
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	==Notes==		==Notes==
	*For the Tris-HCl use Tris base and adjust to desired pH using HCl.		*For the Tris-HCl use Tris base and adjust to desired pH using HCl.
-	* TE buffer is often used to store DNA and RNA. The [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. However, downstream reactions like restriction digests, PCR, ligations, and reverse transcription typically require Mg<sup>2+</sup>, potentially making the presence of EDTA in the reaction problematic. So, when using DNA or RNA that was suspended in TE, you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg<sup>2+</sup> for subsequent reactions to proceed successfully. Each EDTA molecule chelates one Mg<sup>2+</sup> ion.	+	*TE buffer is often used to store DNA and RNA. The [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. However, downstream reactions like restriction digests, PCR, ligations, and reverse transcription typically require Mg<sup>2+</sup>, potentially making the presence of EDTA in the reaction problematic. So, when using DNA or RNA that was suspended in TE, you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg<sup>2+</sup> for subsequent reactions to proceed successfully. Each EDTA molecule chelates one Mg<sup>2+</sup> ion.
	*Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.		*Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.
	*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.		*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.
-	*For ~~dilalution~~ of primers ~~Water for Injection(~~water ~~that is used~~ for ~~dialute~~ injections)can be used ~~effectively~~ rather than ~~going for~~ TE ~~and also for PCR as well~~.	+	*For dilution of primers water for injections can be used rather than TE.

	==See also==		==See also==

Jakob Suckale: /* Purpose */

2009-10-07T17:30:04Z

Purpose

←Older revision		Revision as of 17:30, 7 October 2009
Line 1:		Line 1:
	==Purpose==		==Purpose==
-	TE buffer is often used to store DNA and RNA. ~~The~~ [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.	+	TE buffer is often used to store DNA and RNA.
		+	* [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.
		+	* Tris is a buffering agent to keep the solution at a defined pH.

	==Recipe 10x TE ==		==Recipe 10x TE ==

Jakob Suckale: categories

2009-10-07T17:16:24Z

Jakob Suckale: see also

2009-10-07T17:16:00Z

Jakob Suckale: 1x TE recipe corrected

2009-10-07T17:05:34Z

1x TE recipe corrected

←Older revision		Revision as of 17:05, 7 October 2009
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	\|}		\|}

		+	==Recipe 1x TE ==
		+
		+	{\| {{table}}
		+	\|+ '''for 1 liter of 1x TE solution'''
		+	\|-
		+	\|volume \|\| reagent \|\| final conc.
		+	\|-
		+	\|10 ml \|\| 1M Tris-HCl pH 7.5 or 8.0 (see notes) \|\| 10 mM
		+	\|-
		+	\|2 ml \|\| 0.5M EDTA pH 8.0 \|\| 1 mM
		+	\|-
		+	\|988 ml \|\| ddH<sub>2</sub>O \|\|
		+	\|}

	→ 1x TE is 10 mM [[Tris]]-HCl and 1 mM [[EDTA]]		→ 1x TE is 10 mM [[Tris]]-HCl and 1 mM [[EDTA]]

Jakob Suckale: 10x TE recipe corrected

2009-10-07T17:05:02Z

10x TE recipe corrected

←Older revision		Revision as of 17:05, 7 October 2009
Line 2:		Line 2:
	TE buffer is often used to store DNA and RNA. The [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.		TE buffer is often used to store DNA and RNA. The [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.

-	==Recipe==	+	==Recipe 10x TE ==

-	{\|~~border="1"~~	+	{\| {{table}}
-	\|+ '''~~10xTE'''~~	+	\|+ '''for 1 liter of 10x TE stock solution'''
-	! for 1 liter ~~from~~ stock ~~solutions~~	+
	\|-		\|-
-	\|~~10 ml 1M Tris-HCl pH 7~~.~~5 or 8.0 (see notes)~~	+	\|volume \|\| reagent \|\| final conc.
	\|-		\|-
-	\|2 ml 0.~~5M EDTA pH~~ 8.0	+	\|100 ml \|\| 1M Tris-HCl pH 7.5 or 8.0 (see notes) \|\| 100 mM
	\|-		\|-
-	\|~~988~~ ml ddH<sub>2</sub>O	+	\|20 ml \|\| 0.5M EDTA pH 8.0 \|\| 10 mM
		+	\|-
		+	\|880 ml \|\| ddH<sub>2</sub>O \|\|
	\|}		\|}

-
-	~~{\|border="1"~~
-	~~\|+ '''1xTE'''~~
-	~~! for 1 liter from stock solutions~~
-	\|-
-	~~\|1 ml 1M Tris-HCl pH 7.5 or 8.0 (see notes)~~
-	\|-
-	~~\|0.2 ml 0.5M EDTA pH 8.0~~
-	\|-
-	~~\|988 ml ddH<sub>2</sub>O~~
-	\|}

	→ 1x TE is 10 mM [[Tris]]-HCl and 1 mM [[EDTA]]		→ 1x TE is 10 mM [[Tris]]-HCl and 1 mM [[EDTA]]

R.M.P.D.B Gokarella: /* Notes */

2009-06-25T07:42:11Z

Notes

←Older revision		Revision as of 07:42, 25 June 2009
Line 35:		Line 35:
	*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.		*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.
	*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well.		*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well.
		+
	[[Category:Material]] and [[Category:Buffers]]		[[Category:Material]] and [[Category:Buffers]]

R.M.P.D.B Gokarella: /* Notes */

2009-06-25T07:41:34Z

Notes

←Older revision		Revision as of 07:41, 25 June 2009
Line 34:		Line 34:
	*Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.		*Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.
	*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.		*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.
-	*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well~~.They are completely deionized~~.	+	*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well.
	[[Category:Material]] and [[Category:Buffers]]		[[Category:Material]] and [[Category:Buffers]]

R.M.P.D.B Gokarella: /* Notes */

2009-06-25T07:41:02Z

Notes

←Older revision		Revision as of 07:41, 25 June 2009
Line 34:		Line 34:
	*Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.		*Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.
	*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.		*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.
-	*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well.They are completely deionized ~~and 100pure~~.	+	*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well.They are completely deionized.
	[[Category:Material]] and [[Category:Buffers]]		[[Category:Material]] and [[Category:Buffers]]

←Older revision		Revision as of 17:16, 7 October 2009
Line 43:		Line 43:
	* [http://www.cytographica.com/lab/solutions/TE.htm 10x and 1x TE at cytographica]		* [http://www.cytographica.com/lab/solutions/TE.htm 10x and 1x TE at cytographica]

-	[[Category:Material]] ~~and~~ [[Category:Buffers]]	+
		+	[[Category:Material]]
		+	[[Category:Buffers]]

←Older revision		Revision as of 17:16, 7 October 2009
Line 38:		Line 38:
	*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.		*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.
	*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well.		*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well.
		+
		+	==See also==
		+	* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8018 10x TE buffer at Cold Spring Harbor Protocols]
		+	* [http://www.cytographica.com/lab/solutions/TE.htm 10x and 1x TE at cytographica]

	[[Category:Material]] and [[Category:Buffers]]		[[Category:Material]] and [[Category:Buffers]]