TE

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==Notes==
==Notes==
*Use Tris base and adjust the pH to 8.0 using HCl.
*Use Tris base and adjust the pH to 8.0 using HCl.
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*TE buffer is often used to store DNA.  However, you should realize that the EDTA in TE can chelate the Mg<sup>2+</sup> ions necessary for many reactions such as restriction digests and PCR.  So when using DNA that was suspended in TE you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg<sup>2+</sup> for your reaction to proceed successfully.
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*TE buffer is often used to store DNA.  The EDTA in TE chelates Mg<sup>2+</sup> ions necessary for most processes causing DNA degradation, and thus any residual DNA degradation activity will be suppressed by the presence of EDTA.  However, EDTA, for the same reason, also prevents restriction digests, PCR, and ligations.  So when using DNA that was suspended in TE you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg<sup>2+</sup> for your reaction to proceed successfully.

Revision as of 17:39, 14 November 2005

10X pH 8.0 Recipe

  • 100mM Tris-Cl (pH 8.0)
  • 10mM EDTA (pH 8.0)

Notes

  • Use Tris base and adjust the pH to 8.0 using HCl.
  • TE buffer is often used to store DNA. The EDTA in TE chelates Mg2+ ions necessary for most processes causing DNA degradation, and thus any residual DNA degradation activity will be suppressed by the presence of EDTA. However, EDTA, for the same reason, also prevents restriction digests, PCR, and ligations. So when using DNA that was suspended in TE you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg2+ for your reaction to proceed successfully.
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