TE

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Current revision (09:56, 24 January 2013) (view source)
 
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TE is a shorter name for "Tris-EDTA". For the rest of the story, see [[TAE]], [[Tris]], and [[TBE]].
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==Purpose==
==Purpose==
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TE buffer is often used to store DNA and RNA. The [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.  
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* TE buffer is often used to store DNA and RNA.  
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* [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.
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* [[Tris]] is a buffering agent to keep the solution at a defined pH.
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==Recipe==
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==Recipe 10x TE ==
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{|border="1"
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{| {{table}}
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|+ '''10xTE'''
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|+ '''for 1 liter of 10x TE stock solution'''
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for 1 liter from stock solutions
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|-
|-
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|10 ml 1M Tris-HCl pH 7.5 or 8.0 (see notes) 
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|volume || reagent || final conc.
|-
|-
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|2 ml 0.5M EDTA pH 8.0
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|100 ml || 1M Tris-HCl pH 7.5 or 8.0 (see notes) || 100 mM
|-
|-
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|988 ml ddH<sub>2</sub>O
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|20 ml || 0.5M EDTA pH 8.0 || 10 mM
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|-
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|880 ml || ddH<sub>2</sub>O ||
|}
|}
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==Recipe 1x TE ==
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{|border="1"
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{| {{table}}
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|+ '''1xTE'''
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|+ '''for 1 liter of 1x TE solution'''
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!  for 1 liter from stock solutions
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|-
|-
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|1 ml 1M Tris-HCl pH 7.5 or 8.0 (see notes)
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|volume || reagent || final conc.
|-
|-
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|0.2 ml 0.5M EDTA pH 8.0
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|10 ml || 1M Tris-HCl pH 7.5 or 8.0 (see notes) || 10 mM
|-
|-
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|988 ml ddH<sub>2</sub>O
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|2 ml  || 0.5M EDTA pH 8.0 || 1 mM
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|-
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|988 ml || ddH<sub>2</sub>O ||
|}
|}
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==Notes==
==Notes==
*For the Tris-HCl use Tris base and adjust to desired pH using HCl.
*For the Tris-HCl use Tris base and adjust to desired pH using HCl.
-
* TE buffer is often used to store DNA and RNA. The [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. However, downstream reactions like restriction digests, PCR, ligations, and reverse transcription typically require Mg<sup>2+</sup>, potentially making the presence of EDTA in the reaction problematic.  So, when using DNA or RNA that was suspended in TE, you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg<sup>2+</sup> for subsequent reactions to proceed successfully. Each EDTA molecule chelates one Mg<sup>2+</sup> ion.
+
*TE buffer is often used to store DNA and RNA. The [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. However, downstream reactions like restriction digests, PCR, ligations, and reverse transcription typically require Mg<sup>2+</sup>, potentially making the presence of EDTA in the reaction problematic.  So, when using DNA or RNA that was suspended in TE, you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg<sup>2+</sup> for subsequent reactions to proceed successfully. Each EDTA molecule chelates one Mg<sup>2+</sup> ion.
*Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.
*Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.
*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.
*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.
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*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well.
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*For dilution of primers water for injections can be used rather than TE.
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[[Category:Material]] and [[Category:Buffers]]
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==See also==
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* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8018 10x TE buffer at Cold Spring Harbor Protocols]
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* [http://www.cytographica.com/lab/solutions/TE.htm 10x and 1x TE at cytographica]
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* http://en.wikipedia.org/wiki/TE_buffer
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[[Category:Material]]
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[[Category:Buffers]]

Current revision

TE is a shorter name for "Tris-EDTA". For the rest of the story, see TAE, Tris, and TBE.

Contents

Purpose

  • TE buffer is often used to store DNA and RNA.
  • EDTA in TE chelates Mg2+ and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.
  • Tris is a buffering agent to keep the solution at a defined pH.

Recipe 10x TE

for 1 liter of 10x TE stock solution
volume reagent final conc.
100 ml 1M Tris-HCl pH 7.5 or 8.0 (see notes) 100 mM
20 ml 0.5M EDTA pH 8.0 10 mM
880 ml ddH2O

Recipe 1x TE

for 1 liter of 1x TE solution
volume reagent final conc.
10 ml 1M Tris-HCl pH 7.5 or 8.0 (see notes) 10 mM
2 ml 0.5M EDTA pH 8.0 1 mM
988 ml ddH2O

→ 1x TE is 10 mM Tris-HCl and 1 mM EDTA

Notes

  • For the Tris-HCl use Tris base and adjust to desired pH using HCl.
  • TE buffer is often used to store DNA and RNA. The EDTA in TE chelates Mg2+ and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. However, downstream reactions like restriction digests, PCR, ligations, and reverse transcription typically require Mg2+, potentially making the presence of EDTA in the reaction problematic. So, when using DNA or RNA that was suspended in TE, you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg2+ for subsequent reactions to proceed successfully. Each EDTA molecule chelates one Mg2+ ion.
  • Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.
  • Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions.
  • For dilution of primers water for injections can be used rather than TE.

See also

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