TBS

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(New page: ==Purpose== * TBS stands for tris-buffered saline and is used in many protocols for washing ==Recipe== *Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl {|border="1" |+ '''...)
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*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again  
*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again  
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*'''TBST''': For some applications one adds Tween 20 to TBS which makes TBST.
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**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
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==Contact==
 
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*Who has experience using this material?
 
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Add any other categories that may be relevant to your page.  We use categories like tags.
 
[[Category:Material]] [[Category:Buffers]]
[[Category:Material]] [[Category:Buffers]]

Revision as of 11:43, 2 April 2008

Purpose

  • TBS stands for tris-buffered saline and is used in many protocols for washing


Recipe

  • Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl
5xTBS
for 1 liter with stock solutions
100 ml 1M Tris-HCl pH 7.5
150 ml 5M NaCl
750 ml ddH2O
  • Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again
  • TBST: For some applications one adds Tween 20 to TBS which makes TBST.
    • Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
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