TBE - Revision history

Christopher C Vanlang: /* Purpose */

Christopher C Vanlang — Sat, 07 Jul 2012 02:10:22 GMT

Purpose

←Older revision		Revision as of 02:10, 7 July 2012
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	*'''TBE''' or '''Tris/Borate/EDTA''' is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.		*'''TBE''' or '''Tris/Borate/EDTA''' is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.

-	In molecular biology, TBE and [[TAE ~~buffer\|TAE]]~~ buffers are often used in procedures involving [[nucleic acids]], the most common being [[electrophoresis]]. [[Tris]]-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a ~~[[Chelation\|~~chelator]] of divalent [[cations]], particularly of [[magnesium]] (Mg<sup>2+</sup>). As these ions are necessary co-factors for many enzymes, including contaminant [[nucleases]], the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg<sup>2+</sup> is also a co-factor for many useful DNA-modifying enzymes such as [[restriction enzyme]]s and [[DNA polymerase]]s, its concentration in TBE or TAE buffers is generally kept low (typically at around 1 ~~[[Concentration#Molarity\|~~mM]]).	+	In molecular biology, TBE and TAE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg<sup>2+</sup>). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg<sup>2+</sup> is also a co-factor for many useful DNA-modifying enzymes such as [[restriction enzyme]]s and [[DNA polymerase]]s, its concentration in TBE or TAE buffers is generally kept low (typically at around 1 mM).

	==Recipe==		==Recipe==

Christopher C Vanlang at 02:09, 7 July 2012

Christopher C Vanlang — Sat, 07 Jul 2012 02:09:00 GMT

←Older revision		Revision as of 02:09, 7 July 2012
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	==Purpose==		==Purpose==
	*'''TBE''' or '''Tris/Borate/EDTA''' is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.		*'''TBE''' or '''Tris/Borate/EDTA''' is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.
		+
		+	In molecular biology, TBE and [[TAE buffer\|TAE]] buffers are often used in procedures involving [[nucleic acids]], the most common being [[electrophoresis]]. [[Tris]]-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a [[Chelation\|chelator]] of divalent [[cations]], particularly of [[magnesium]] (Mg<sup>2+</sup>). As these ions are necessary co-factors for many enzymes, including contaminant [[nucleases]], the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg<sup>2+</sup> is also a co-factor for many useful DNA-modifying enzymes such as [[restriction enzyme]]s and [[DNA polymerase]]s, its concentration in TBE or TAE buffers is generally kept low (typically at around 1 [[Concentration#Molarity\|mM]]).

	==Recipe==		==Recipe==
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	#Adjust volume to 1 Liter.		#Adjust volume to 1 Liter.
	#Store at room temperature.		#Store at room temperature.
		+	'''Note:''' 0.5x TBE may also be used for electrophoresis.

	==Commercial sources==		==Commercial sources==

Christopher C Vanlang at 02:07, 7 July 2012

Christopher C Vanlang — Sat, 07 Jul 2012 02:07:15 GMT

←Older revision		Revision as of 02:07, 7 July 2012
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	==Purpose==		==Purpose==
-	*TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.	+	*'''TBE''' or '''Tris/Borate/EDTA''' is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.

	==Recipe==		==Recipe==

Torsten Waldminghaus at 07:09, 7 May 2008

Torsten Waldminghaus — Wed, 07 May 2008 07:09:15 GMT

←Older revision		Revision as of 07:09, 7 May 2008
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	#Adjust volume to 1 Liter.		#Adjust volume to 1 Liter.
	#Store at room temperature.		#Store at room temperature.
		+	'''Note:''' 10x TBE may take some time to dissolve, even with fast stirring

	'''1x TBE (1 liter):'''		'''1x TBE (1 liter):'''

Torsten Waldminghaus at 07:06, 7 May 2008

Torsten Waldminghaus — Wed, 07 May 2008 07:06:57 GMT

←Older revision		Revision as of 07:06, 7 May 2008
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		+	==Purpose==
		+	*TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.
		+
		+	==Recipe==
		+	'''10x TBE (1 liter):'''
	#Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.		#Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.
-	#Add 40 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0)	+	#Add 40 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0) (alternatively use 9.3 g Na<sub>2</sub>EDTA)
		+	#Adjust volume to 1 Liter.
		+	#Store at room temperature.
		+
		+	'''1x TBE (1 liter):'''
		+	#Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water.
		+	#Add 4 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0)
	#Adjust volume to 1 Liter.		#Adjust volume to 1 Liter.
	#Store at room temperature.		#Store at room temperature.

Torsten Waldminghaus: 10X TBE moved to TBE

Torsten Waldminghaus — Wed, 07 May 2008 07:01:01 GMT

10X TBE moved to TBE

←Older revision

Revision as of 07:01, 7 May 2008

Torsten Waldminghaus at 14:19, 2 April 2008

Torsten Waldminghaus — Wed, 02 Apr 2008 14:19:43 GMT

←Older revision		Revision as of 14:19, 2 April 2008
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	#[https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=195&catname=North%20America%20Main UltraPure™ 10X TBE Buffer] from Invitrogen		#[https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=195&catname=North%20America%20Main UltraPure™ 10X TBE Buffer] from Invitrogen

-	[[Category:Material]] [[Category:Agarose gel electrophoresis]][[Buffers]]	+	[[Category:Material]] [[Category:Agarose gel electrophoresis]][[Category:Buffers]]

Torsten Waldminghaus: add link

Torsten Waldminghaus — Wed, 02 Apr 2008 14:19:11 GMT

add link

←Older revision		Revision as of 14:19, 2 April 2008
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	#[https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=195&catname=North%20America%20Main UltraPure™ 10X TBE Buffer] from Invitrogen		#[https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=195&catname=North%20America%20Main UltraPure™ 10X TBE Buffer] from Invitrogen

-	[[Category:Material]] [[Category:Agarose gel electrophoresis]]	+	[[Category:Material]] [[Category:Agarose gel electrophoresis]][[Buffers]]

Torsten Waldminghaus: add tags

Torsten Waldminghaus — Tue, 11 Mar 2008 15:33:09 GMT

add tags

←Older revision		Revision as of 15:33, 11 March 2008
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	==Commercial sources==		==Commercial sources==
	#[https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=195&catname=North%20America%20Main UltraPure™ 10X TBE Buffer] from Invitrogen		#[https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=195&catname=North%20America%20Main UltraPure™ 10X TBE Buffer] from Invitrogen
		+
		+	[[Category:Material]] [[Category:Agarose gel electrophoresis]]

Reshma P. Shetty at 00:41, 14 December 2006

Reshma P. Shetty — Thu, 14 Dec 2006 00:41:32 GMT

←Older revision		Revision as of 00:41, 14 December 2006
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	#Adjust volume to 1 Liter.		#Adjust volume to 1 Liter.
	#Store at room temperature.		#Store at room temperature.
		+
		+	==Commercial sources==
		+	#[https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=195&catname=North%20America%20Main UltraPure™ 10X TBE Buffer] from Invitrogen