TBE

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 8: Line 8:
#Adjust volume to 1 Liter.
#Adjust volume to 1 Liter.
#Store at room temperature.
#Store at room temperature.
 +
'''Note:''' 10x TBE may take some time to dissolve, even with fast stirring
'''1x TBE (1 liter):'''
'''1x TBE (1 liter):'''

Revision as of 03:09, 7 May 2008

Purpose

  • TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.

Recipe

10x TBE (1 liter):

  1. Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.
  2. Add 40 ml 0.5 M Na2EDTA (pH 8.0) (alternatively use 9.3 g Na2EDTA)
  3. Adjust volume to 1 Liter.
  4. Store at room temperature.

Note: 10x TBE may take some time to dissolve, even with fast stirring

1x TBE (1 liter):

  1. Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water.
  2. Add 4 ml 0.5 M Na2EDTA (pH 8.0)
  3. Adjust volume to 1 Liter.
  4. Store at room temperature.

Commercial sources

  1. UltraPure™ 10X TBE Buffer from Invitrogen
Personal tools