TBE

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m (10X TBE moved to TBE)
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==Purpose==
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*TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.
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==Recipe==
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'''10x TBE (1 liter):'''
#Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.  
#Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.  
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#Add 40 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0)
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#Add 40 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0) (alternatively use 9.3 g Na<sub>2</sub>EDTA)
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#Adjust volume to 1 Liter.
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#Store at room temperature.
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'''1x TBE (1 liter):'''
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#Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water.
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#Add 4 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0)  
#Adjust volume to 1 Liter.
#Adjust volume to 1 Liter.
#Store at room temperature.
#Store at room temperature.

Revision as of 02:06, 7 May 2008

Purpose

  • TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.

Recipe

10x TBE (1 liter):

  1. Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.
  2. Add 40 ml 0.5 M Na2EDTA (pH 8.0) (alternatively use 9.3 g Na2EDTA)
  3. Adjust volume to 1 Liter.
  4. Store at room temperature.

1x TBE (1 liter):

  1. Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water.
  2. Add 4 ml 0.5 M Na2EDTA (pH 8.0)
  3. Adjust volume to 1 Liter.
  4. Store at room temperature.

Commercial sources

  1. UltraPure™ 10X TBE Buffer from Invitrogen
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