TAE

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TAE is a commonly used buffer for making and running DNA agarose gels.
TAE is a commonly used buffer for making and running DNA agarose gels.
It offers a few advantages and disadvantages compared to TBE buffer:
It offers a few advantages and disadvantages compared to TBE buffer:
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*TAE buffer provides optimal resolution of fragments >4 kb in length, while TBE provides better resolution for 0.1 to 3 kb fragments.
* TAE is significantly cheaper to make
* TAE is significantly cheaper to make
* TAE stocks can be 50X concentrated and therefore take up less space than 10X concentrated TBE stock
* TAE stocks can be 50X concentrated and therefore take up less space than 10X concentrated TBE stock

Revision as of 15:35, 5 August 2011

TAE is a commonly used buffer for making and running DNA agarose gels. It offers a few advantages and disadvantages compared to TBE buffer:

  • TAE buffer provides optimal resolution of fragments >4 kb in length, while TBE provides better resolution for 0.1 to 3 kb fragments.
  • TAE is significantly cheaper to make
  • TAE stocks can be 50X concentrated and therefore take up less space than 10X concentrated TBE stock
  • TBE offers a higher resolution and has a higher buffering capacity at greater temperatures induced by relatively higher voltages
  • TBE can negatively influence the yield of DNA after recovery from a gel when using glass-based protocols. Yield does not seem to be influenced when using silica-based methods.
  • The bromide ion in TBE is a powerful inhibitor of enzyme activity. This can prevent degradation of nucleic acid, but can also interfere with subsequent experiments like cloning DNA into a vector.

Ingredients for one litre 50X stock

  • Tris-base: 242 g
  • Acetate (100% acetic acid): 57.1 ml
  • EDTA: 100 ml 0.5M sodium EDTA

Add dH2O up to one litre.

To make 1x TAE from 50X TAE stock, dilute 20ml of stock into 980 ml of deionised water. and

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