T4 DNA ligase: Difference between revisions
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==Commercial Sources== | ==Commercial Sources== | ||
* [http://www.neb.com/nebecomm/products/productM0202.asp NEB] | * [http://www.neb.com/nebecomm/products/productM0202.asp NEB] | ||
==Notes== | |||
* T4 DNA ligase is very sensitive to salt concentration. Greater than 70mM NaCl can begin to have inhibitory effect and 200mM NaCl can completely inactivate the enzyme. Even though NEB says you can use the ligase in all NEBuffers, NEB3 contains 100mM NaCl and has been found to not work too well. --[[User:Austin J. Che|Austin Che]] 17:03, 17 September 2007 (EDT) | |||
Revision as of 14:03, 17 September 2007
T4 DNA ligase is often used for DNA ligation.
Commercial Sources
Notes
- T4 DNA ligase is very sensitive to salt concentration. Greater than 70mM NaCl can begin to have inhibitory effect and 200mM NaCl can completely inactivate the enzyme. Even though NEB says you can use the ligase in all NEBuffers, NEB3 contains 100mM NaCl and has been found to not work too well. --Austin Che 17:03, 17 September 2007 (EDT)
