Synthetic Society/SB Reality: Difference between revisions
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==Construction Technology== | ==Construction Technology== | ||
Conclusion: It is possible to construct any piece of DNA.<br> | |||
<br> | |||
Characteristics as of 1 February 2006: | |||
Cost. ~$1 per bp | |||
Length. ~100 bp direct chemical synthesis, ~15,000 bp PCR assembly, ~3,500,000 bp inchworm elongation | |||
Error Rate. From 1:10 to effectively zero | |||
Time. 6 hours for 100 bp, 2-6 weeks for 15,000 bp, ~2 months for 50,000 bp, ~7 years for 3,500,000 bp | |||
Diversity. From one molecule to millions of molecules (heavily dependent on method) | |||
Terms of Sale. Varied (from no-strings-attached to full-rights-reserved). | |||
Examples: | |||
*1918 Flu resurrection | *1918 Flu resurrection | ||
*Refactoring Bacteriophage T7 | *Refactoring Bacteriophage T7 | ||
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*Two genomes, one cell | *Two genomes, one cell | ||
*Stemmer | *Stemmer | ||
*Baric | |||
==Ownership, Sharing, & Innovation Technology== | ==Ownership, Sharing, & Innovation Technology== | ||
Revision as of 08:25, 1 February 2006
Construction Technology
Conclusion: It is possible to construct any piece of DNA.
Characteristics as of 1 February 2006:
Cost. ~$1 per bp
Length. ~100 bp direct chemical synthesis, ~15,000 bp PCR assembly, ~3,500,000 bp inchworm elongation
Error Rate. From 1:10 to effectively zero
Time. 6 hours for 100 bp, 2-6 weeks for 15,000 bp, ~2 months for 50,000 bp, ~7 years for 3,500,000 bp
Diversity. From one molecule to millions of molecules (heavily dependent on method)
Terms of Sale. Varied (from no-strings-attached to full-rights-reserved).
Examples:
- 1918 Flu resurrection
- Refactoring Bacteriophage T7
- Poliovirus synthesis
- PhiX174 synthesis
- E.coli camera
- Programmed pattern formation
- Venter at NSABB
- Two genomes, one cell
- Stemmer
- Baric
Ownership, Sharing, & Innovation Technology
- MTA examples
- BioBrick examples
