Synthetic Biology:Vectors/Wishlist

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This is a wishlist of vectors that we would like to have but are not currently available.

pSB2*

pSB2AT3-P1010

purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.

pSB2AK3-P1010

purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.

pSB2AC3-P1010

purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.

pSB4*

Randy would like to have the full complement of low copy vectors for assembly purposes.

Here is what they might look like using a pSC101 origin:

5' BBa_P1011 (ccdB) --

BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_B0045 (NheI) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_B0046 (NsiI) -- BBa_B0047 (MfeI) -- BBa_I50041 (pSC101, reverse) -- BBa_B0048 (AvrII) --

BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3'

pSB5*

A single copy plasmid for characterization and operation of devices and systems.

5' BBa_P1011 (ccdB) -- BBa_I50020 (hc ori)--

BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_B0045 (NheI) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_B0046 (NsiI) -- BBa_B0047 (MfeI) -- BBa_I50001 (F plasmid, reverse) -- BBa_B0048 (AvrII) --

BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3'

pSB6*

Randy would like to have the full complement of low copy vectors for assembly purposes.

Here is what they might look like using a pBR322 origin:

5' BBa_P1011 (ccdB) --

BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_B0045 (NheI) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_B0046 (NsiI) -- BBa_B0047 (MfeI) -- BBa_I50030 (pBR322, switch orientation) -- BBa_B0048 (AvrII) --

BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3'

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