Synthetic Biology:Vectors/Wishlist

This is a wishlist of vectors that we would like to have but are not currently available.

pSB2*

pSB2AT3-P1010

purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.

pSB2AK3-P1010

purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.

pSB2AC3-P1010

purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.

pSB4*

Randy would like to have the full complement of low copy vectors for assembly purposes.

Here is what they might look like using a pSC101 origin:

5' BBa_P1010 (ccd) --

BBsuffix -- TOPO site -- translational stop sequence -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50040 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) --

BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- translational stop sequence -- TOPO site -- BB prefix 3'

pSB5*

A single copy plasmid for characterization and operation of devices and systems.

pSB6*

Randy would like to have the full complement of low copy vectors for assembly purposes.

Here is what they might look like using a pBR322 origin:

5' BBa_P1010 (ccd) --

BBsuffix -- TOPO site -- translational stop sequence -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50030 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) --

BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- translational stop sequence -- TOPO site -- BB prefix 3'