Synthetic Biology:Vectors/Wishlist: Difference between revisions
No edit summary |
No edit summary |
||
| (15 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
===pSB2AT3- | '''This is a wishlist of vectors that we would like to have but are not currently available.''' | ||
==pSB2*== | |||
===pSB2AT3-P1010=== | |||
purpose: inducible copy number plasmid to be used in the three antibiotic selection | purpose: inducible copy number plasmid to be used in the three antibiotic selection | ||
assembly technique. | assembly technique. | ||
===pSB2AK3- | ===pSB2AK3-P1010=== | ||
purpose: inducible copy number plasmid to be used in the three antibiotic selection | purpose: inducible copy number plasmid to be used in the three antibiotic selection | ||
assembly technique. | assembly technique. | ||
===pSB2AC3- | ===pSB2AC3-P1010=== | ||
purpose: inducible copy number plasmid to be used in the three antibiotic selection | purpose: inducible copy number plasmid to be used in the three antibiotic selection | ||
assembly technique. | assembly technique. | ||
==pSB4*== | |||
Randy would like to have the full complement of low copy vectors for assembly purposes. | |||
Here is what they might look like using a pSC101 origin: | |||
<b> | |||
5' BBa_P1011 (ccdB) -- | |||
BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_B0045 (NheI) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_B0046 (NsiI) -- BBa_B0047 (MfeI) -- BBa_I50041 (pSC101, reverse) -- BBa_B0048 (AvrII) -- | |||
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3' | |||
</b> | |||
==pSB5*== | |||
A [[Synthetic Biology:Vectors/Single copy plasmid | single copy plasmid]] for characterization and operation of devices and systems. | |||
<b> | |||
5' BBa_P1011 (ccdB) -- BBa_I50020 (hc ori)-- | |||
BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_B0045 (NheI) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_B0046 (NsiI) -- BBa_B0047 (MfeI) -- BBa_I50001 (F plasmid, reverse) -- BBa_B0048 (AvrII) -- | |||
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3' | |||
</b> | |||
==pSB6*== | |||
Randy would like to have the full complement of low copy vectors for assembly purposes. | |||
Here is what they might look like using a pBR322 origin: | |||
<b> | |||
5' BBa_P1011 (ccdB) -- | |||
BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_B0045 (NheI) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_B0046 (NsiI) -- BBa_B0047 (MfeI) -- BBa_I50030 (pBR322, ''switch orientation'') -- BBa_B0048 (AvrII) -- | |||
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3' | |||
</b> | |||
Latest revision as of 16:13, 13 March 2006
This is a wishlist of vectors that we would like to have but are not currently available.
pSB2*
pSB2AT3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB2AK3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB2AC3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB4*
Randy would like to have the full complement of low copy vectors for assembly purposes.
Here is what they might look like using a pSC101 origin:
5' BBa_P1011 (ccdB) --
BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_B0045 (NheI) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_B0046 (NsiI) -- BBa_B0047 (MfeI) -- BBa_I50041 (pSC101, reverse) -- BBa_B0048 (AvrII) --
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3'
pSB5*
A single copy plasmid for characterization and operation of devices and systems.
5' BBa_P1011 (ccdB) -- BBa_I50020 (hc ori)--
BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_B0045 (NheI) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_B0046 (NsiI) -- BBa_B0047 (MfeI) -- BBa_I50001 (F plasmid, reverse) -- BBa_B0048 (AvrII) --
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3'
pSB6*
Randy would like to have the full complement of low copy vectors for assembly purposes.
Here is what they might look like using a pBR322 origin:
5' BBa_P1011 (ccdB) --
BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_B0045 (NheI) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_B0046 (NsiI) -- BBa_B0047 (MfeI) -- BBa_I50030 (pBR322, switch orientation) -- BBa_B0048 (AvrII) --
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3'
