Synthetic Biology:Vectors/Wishlist: Difference between revisions
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5' BBa_P1010 (ccd) -- | 5' BBa_P1010 (ccd) -- | ||
BBsuffix -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- translational stop sequence -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50040 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) -- | BBsuffix -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- BBa_B0042 (translational stop sequence) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50040 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) -- | ||
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- translational stop sequence -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- BB prefix 3' | BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0042 (translational stop sequence) -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- BB prefix 3' | ||
</b> | </b> | ||
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5' BBa_P1010 (ccd) -- | 5' BBa_P1010 (ccd) -- | ||
BBsuffix -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- translational stop sequence -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50030 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) -- | BBsuffix -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- BBa_B0042 (translational stop sequence) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50030 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) -- | ||
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- translational stop sequence -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- BB prefix 3' | BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0042 (translational stop sequence) -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- BB prefix 3' | ||
</b> | </b> | ||
Revision as of 15:59, 4 February 2006
This is a wishlist of vectors that we would like to have but are not currently available.
pSB2*
pSB2AT3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB2AK3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB2AC3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB4*
Randy would like to have the full complement of low copy vectors for assembly purposes.
Here is what they might look like using a pSC101 origin:
5' BBa_P1010 (ccd) --
BBsuffix -- TOPO site -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50040 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) --
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- TOPO site -- BB prefix 3'
pSB5*
A single copy plasmid for characterization and operation of devices and systems.
pSB6*
Randy would like to have the full complement of low copy vectors for assembly purposes.
Here is what they might look like using a pBR322 origin:
5' BBa_P1010 (ccd) --
BBsuffix -- TOPO site -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50030 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) --
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- TOPO site -- BB prefix 3'
