Synthetic Biology:Vectors/Parts

This is a list of BioBricks parts for use in construction of modular vectors.

Some have just been designed while others have been constructed and tested. See the associated registry page for details.

Replication origins

• <bbpart>BBa_I50000</bbpart>: F plasmid backbone with BioBricks restriction sites removed
• <bbpart>BBa_I50010</bbpart>: oriV origin which requires TrfA protein to be functional.
• <bbpart>BBa_I50020</bbpart>: high copy origin of replication from pSB1A3
• <bbpart>BBa_I50030</bbpart>: a pBR322 origin.
• <bbpart>BBa_I50040</bbpart>: a near minimal pSC101 origin.
• <bbpart>BBa_I50050</bbpart>: a R6K gamma origin. See also EZ-Tn5™ <R6Kγori /KAN-2> Sequence general information (Note: this origin will only replicate in a pir⁺ strain.)

Antibiotic resistance cassettes

• <bbpart>BBa_P1001</bbpart>: cassette providing tetracycline resistance.
• <bbpart>BBa_P1000</bbpart>: cassette providing chloramphenicol resistance.
• <bbpart>BBa_P1002</bbpart>: cassette providing ampicillin resistance.
• <bbpart>BBa_P1003</bbpart>: cassette providing kanamycin resistance.
• <bbpart>BBa_P1004</bbpart>: cassette providing ampicillin resistance in reverse orientation

Terminators

• <bbpart>BBa_B0055</bbpart>: upstream flanking terminator
• <bbpart>BBa_B0054</bbpart>: downstream flanking terminator
• <bbpart>BBa_B0053</bbpart>: bidirectional terminator from E. coli his operon
• <bbpart>BBa_B0052</bbpart>: forward terminator
• <bbpart>BBa_B0062</bbpart>: reverse terminator of BBa_B0052.

Notes

It wasn't clear from the website if these were bi-directional? Not sure that this is very important--BC

I don't know if the terminators are bidirectional. These terminators are used as flanking terminators in other vectors and are claimed to make the cloning of difficult pieces of DNA (like strong promoters) easier. This is why I was planning on orienting the antibiotic resistance cassette in the opposite direction so that read through from upstream of the multiple cloning site is less of an issue. -- RS

Others

• <bbpart> BBa_I1000</bbpart> (old) or <bbpart>BBa_P1010</bbpart> (preferred): ccd operon in BioBricks format
• unique identifier for the vector