Synthetic Biology:Vectors/Nomenclature: Difference between revisions

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==pSBxyz-a==
Please see the [http://parts.mit.edu Registry of Standard Biological Parts] [http://parts.mit.edu/registry/index.php/Help:Plasmids/Nomenclature information on vector nomenclature].  All updates should be made there.
 
pSB = plasmid Synthetic Biology<br>
x = origin of replication<br>
y = selection marker<br>
z = version number<br>
-a = insert
 
===Origin of replication===
*1= pUC19-derived pMB1 (in pSB series)
*2 = pSCANS-derived
*3 = p15A pMR101-derived
*4 = rep101, repA pSC101-derived
 
===Selection marker===
*X = none
*A = ampicillin
*C = chloramphenicol
*K = kanamycin
*T = tetracycline
*N = neomycin
*S = spectinomycin
*St = streptomycin
*R = rifampicin
*Na = nalidixic acid
*AK = kan and amp
*KT = kan and tet
*AKT = amp and kan and tet
*C = cam
*AC= amp and cam
… etc
 
===Version number===
(used to indicate miscellaneous interesting changes to backbone)
*0 = nothing
*1 = complete Biobricks insertion site
*2 = 5’ terminator and complete Biobricks insertion site
*3 = 5’ terminator and complete Biobricks insertion site and 3’ terminator
 
===Insert===
*generally a BioBricks part number where multiple parts are separated by periods
*no dash = no insert
*-1 = [[ccdB]]

Latest revision as of 12:42, 19 October 2006

Please see the Registry of Standard Biological Parts information on vector nomenclature. All updates should be made there.