Synthetic Biology:Vectors/Inventory

pSB1A1

• constructed by Tom Knight

pSB1A2-1

• derived from pSB1A1
• constructed by Leon Chan

pSB1A3-1

• (formerly known as pSB104.2)
• purpose: base plasmid used to insert biobricks parts
• derived from pSB1A2-1
• constructed by Reshma

pSB4A1

• (formerly known as pSC101 v1.1)
• purpose: low copy number plasmid to be used for unstable assemblies that contain lac components.
• derived from Elowitz's repressilator plasmid
• constructed by Reshma

pSB4A3-1

• purpose: low copy number plasmid to be used for unstable assemblies that contain lac components. Contains ccdB to facilitate cloning.
• derived from pSB4A1
• constructed by Reshma

pSB3K1

• (formerly known as p15A-Kan v2.0)
• purpose: low copy number plasmid to be used for unstable assemblies that contain lac components.
• constructed by Reshma

pSB3K3-1

• purpose: low copy number plasmid to be used for unstable assemblies that contain lac components. Contains ccdB to facilitate cloning.
• derived from pSB3K1
• constructed by Reshma

pSB2K0

• (formerly known as pSCANS)
• purpose: inducible copy number plasmid
• problems: contains a PstI site in backbone

pSB2K3-1

• purpose: IPTG-inducible copy number plasmid
• derived from pSB2K0
• constructed by Sri Kosuri?

pSB1AT3-1

• (formerly known as pSB204.3 or pSB3.1.7.1)
• purpose: high copy number plasmid to be used in the three antibiotic selection assembly technique.
• derived from pSB1A3-1
• constructed by Reshma

pSB1AK3-1

• (formerly known as pSB304.3 or pSB5.1.7.1)
• purpose: high copy number plasmid to be used in the three antibiotic selection assembly technique.
• derived from pSB1A3-1
• constructed by Reshma

pSB1AC3-1

• purpose: high copy number plasmid to be used in the three antibiotic selection assembly technique.
• derived from pSB1A3-1
• constructed by Robert Bryant