Synthetic Biology:Vectors/Inventory: Difference between revisions
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(Added more information about origins of the various vectors.) |
(→pSB2K0) |
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*constructed by [[User:Rshetty|Reshma]] | *constructed by [[User:Rshetty|Reshma]] | ||
===pSB2K0=== | ===pSB2K0=== | ||
*( | *(also known as [http://www.genome.bnl.gov/Protocols/pscans.shtml pSCANS]) | ||
*purpose: inducible copy number plasmid | *purpose: inducible copy number plasmid | ||
*problems: contains a PstI site in backbone | *problems: contains a PstI site in backbone | ||
===pSB2K3-1=== | ===pSB2K3-1=== | ||
*purpose: IPTG-inducible copy number plasmid | *purpose: IPTG-inducible copy number plasmid | ||
Revision as of 12:23, 8 August 2005
pSB1A1
- constructed by Tom Knight
pSB1A2-1
- derived from pSB1A1
- constructed by Leon Chan
pSB1A3-1
- (formerly known as pSB104.2)
- purpose: base plasmid used to insert biobricks parts
- derived from pSB1A2-1
- constructed by Reshma
pSB4A1
- (formerly known as pSC101 v1.1)
- purpose: low copy number plasmid to be used for unstable assemblies that contain lac components.
- derived from Elowitz's repressilator plasmid
- constructed by Reshma
pSB4A3-1
- purpose: low copy number plasmid to be used for unstable assemblies that contain lac components. Contains ccdB to facilitate cloning.
- derived from pSB4A1
- constructed by Reshma
pSB3K1
- (formerly known as p15A-Kan v2.0)
- purpose: low copy number plasmid to be used for unstable assemblies that contain lac components.
- constructed by Reshma
pSB3K3-1
- purpose: low copy number plasmid to be used for unstable assemblies that contain lac components. Contains ccdB to facilitate cloning.
- derived from pSB3K1
- constructed by Reshma
pSB2K0
- (also known as pSCANS)
- purpose: inducible copy number plasmid
- problems: contains a PstI site in backbone
pSB2K3-1
- purpose: IPTG-inducible copy number plasmid
- derived from pSB2K0
- constructed by Sri Kosuri?
pSB1AT3-1
- (formerly known as pSB204.3 or pSB3.1.7.1)
- purpose: high copy number plasmid to be used in the three antibiotic selection assembly technique.
- derived from pSB1A3-1
- constructed by Reshma
pSB1AK3-1
- (formerly known as pSB304.3 or pSB5.1.7.1)
- purpose: high copy number plasmid to be used in the three antibiotic selection assembly technique.
- derived from pSB1A3-1
- constructed by Reshma
pSB1AC3-1
- purpose: high copy number plasmid to be used in the three antibiotic selection assembly technique.
- derived from pSB1A3-1
- constructed by Robert Bryant
