SynBioBootcamp:Notebook/Shane Peralta: Difference between revisions

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==Entry 1: 10 February 2011==
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Hai gais, amidoinitrite?
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|colspan="2" style="background-color: #F2F2F2;" align="right"|[[{{FULLPAGENAME}}/Entry_Base|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
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==Project Description/Abstract==
* Place short description of project or notes regarding this project


* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
==Entry 2: 15 February 2011==
Today I...
* Set up all my oligos/templates/buffers in my pcr tubes. I pretty much just followed the first few parts of the PCR cloning protocol, to the letter.


==Notes==
==Entry 3: 17 February 2011==
* Place some notes here for visitors
Today I...
* Ran my PCR products on an analytical gel (you know, just to check). Everything was as expected.
* Did a regular Zymo cleanup on my products. I fear that I may have mislabeled one of my tubes since I had 2 that said sbb1113 and none that said sbb1119. I just changed one to 1119. Let's hope for the best.


* Example: This project is currently on hold until further notice.
==Entry 4: 22 February 2011==
It looks like someone gave us the wrong buffer or something, according to the prof. I guess he redid everyones products. Lucky!


Anyway, today I...
* Digested my products with EcoRI and BamHI, according to the digest protocol.
* Ran a gel and then cut my products out of it. That was pretty interesting, but I think I spent too much time in the UV because my eyes kinda hurt.


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==Entry 5: 24 February 2011==
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Today I...
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* Ran a Zymo gel purification, according to protocol.
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__NOTOC__
==Entry 6: 1 March 2011==
Today I...
* Ligated my digests into plasmids.
* Transformed bacteria with the plasmids. Yay! It's like I've got 3 plates full of bacterial pets. I hope they grow up big and strong.
 
==Entry 7: 3 March 2011==
Today I...
* Did a miniprep. Originally I had 12 good colonies (4 vials for each part), but 2 of my 1113s turned pink after I pelleted. Too bad. Also, I had to kill all the bacteria that grew in the vials. So sad. Oh well, they gave up their lives for a greater purpose: my project.
 
==Entry 8: 8 March 2011==
I showed up to class an hour-and-a-half late. Stupid alarm.
 
Anyway, today I...

Latest revision as of 13:05, 8 March 2011

Entry 1: 10 February 2011

Hai gais, amidoinitrite?

Entry 2: 15 February 2011

Today I...

  • Set up all my oligos/templates/buffers in my pcr tubes. I pretty much just followed the first few parts of the PCR cloning protocol, to the letter.

Entry 3: 17 February 2011

Today I...

  • Ran my PCR products on an analytical gel (you know, just to check). Everything was as expected.
  • Did a regular Zymo cleanup on my products. I fear that I may have mislabeled one of my tubes since I had 2 that said sbb1113 and none that said sbb1119. I just changed one to 1119. Let's hope for the best.

Entry 4: 22 February 2011

It looks like someone gave us the wrong buffer or something, according to the prof. I guess he redid everyones products. Lucky!

Anyway, today I...

  • Digested my products with EcoRI and BamHI, according to the digest protocol.
  • Ran a gel and then cut my products out of it. That was pretty interesting, but I think I spent too much time in the UV because my eyes kinda hurt.

Entry 5: 24 February 2011

Today I...

  • Ran a Zymo gel purification, according to protocol.

Entry 6: 1 March 2011

Today I...

  • Ligated my digests into plasmids.
  • Transformed bacteria with the plasmids. Yay! It's like I've got 3 plates full of bacterial pets. I hope they grow up big and strong.

Entry 7: 3 March 2011

Today I...

  • Did a miniprep. Originally I had 12 good colonies (4 vials for each part), but 2 of my 1113s turned pink after I pelleted. Too bad. Also, I had to kill all the bacteria that grew in the vials. So sad. Oh well, they gave up their lives for a greater purpose: my project.

Entry 8: 8 March 2011

I showed up to class an hour-and-a-half late. Stupid alarm.

Anyway, today I...

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