Switchgrass Lab Protocols: Difference between revisions
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==Diploid ''Panicum'' crossing techniques== | |||
Crossing the diploid ''Panicum'' species ''P. hallii'', ''P. filipes'', and ''P. capillare'' is difficult because the species are strongly inclined to self-fertilize. In order to make successful crosses, hand emasculation must be used. Because flowers are small I have always used a dissecting microscope. | |||
The method I have found most successful is the following: | |||
1) Place plants in the dark over night | |||
2) Remove plants from dark the next morning around 8-10am | |||
3) Search for flowers that look as if they are about to open (i.e. lemma and palea starting to separate) | |||
4) Maneuver plant so that flower is under dissecting microscope | |||
5) | |||
==Microsat Primers== | ==Microsat Primers== | ||
Revision as of 10:10, 18 March 2011
Diploid Panicum crossing techniques
Crossing the diploid Panicum species P. hallii, P. filipes, and P. capillare is difficult because the species are strongly inclined to self-fertilize. In order to make successful crosses, hand emasculation must be used. Because flowers are small I have always used a dissecting microscope.
The method I have found most successful is the following:
1) Place plants in the dark over night 2) Remove plants from dark the next morning around 8-10am 3) Search for flowers that look as if they are about to open (i.e. lemma and palea starting to separate) 4) Maneuver plant so that flower is under dissecting microscope 5)
Microsat Primers
Coming soon
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