Swartz:Research/Biohydrogen: Difference between revisions
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The first major goal of this research was the evolution of an oxygen-tolerant [FeFe] hydrogenase. This has proven to be extremely difficult; indeed many other research groups have tried this and been ultimately unsuccessful. However, our evolution methodology [papers] is different and has led to some level of success in this endeavor [paper]; however, much remains to be done to achieve this overall objective. | The first major goal of this research was the evolution of an oxygen-tolerant [FeFe] hydrogenase. This has proven to be extremely difficult; indeed many other research groups have tried this and been ultimately unsuccessful. However, our evolution methodology [papers] is different and has led to some level of success in this endeavor [paper]; however, much remains to be done to achieve this overall objective. | ||
Bingham AS, Smith PR, Swartz JR. Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity. ''International Journal of Hydrogen Energy'' (2011) [http://dx.doi.org/10.1016/j.ijhydene.2011.02.048 doi:10.1016/j.ijhydene.2011.02.048] | *Bingham AS, Smith PR, Swartz JR. Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity. ''International Journal of Hydrogen Energy'' (2011) [http://dx.doi.org/10.1016/j.ijhydene.2011.02.048 doi:10.1016/j.ijhydene.2011.02.048] | ||
The original vision was that such a hydrogenase, once evolved, would be used in a photosynthetic microorganism to produce hydrogen directly from sunlight and water. This would require metabolic engineering of an insulated electron transfer circuit from the photosystems to the hydrogenase [papers, presentations] | The original vision was that such a hydrogenase, once evolved, would be used in a photosynthetic microorganism to produce hydrogen directly from sunlight and water. This would require metabolic engineering of an insulated electron transfer circuit from the photosystems to the hydrogenase [papers, presentations] | ||
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A second project was begun in 2009 focusing on the production of hydrogen from biomass. The approach is to build a synthetic enzyme pathway that is able to produce hydrogen from NADPH; this pathway would then be coupled to the pentose phosphate pathway to enable hydrogen production from glucose (a proxy for biomass). [papers, presentations] | A second project was begun in 2009 focusing on the production of hydrogen from biomass. The approach is to build a synthetic enzyme pathway that is able to produce hydrogen from NADPH; this pathway would then be coupled to the pentose phosphate pathway to enable hydrogen production from glucose (a proxy for biomass). [papers, presentations] | ||
Smith PR, Bingham AS, Swartz JR. Generation of hydrogen from NADPH using an [FeFe] hydrogenase. ''International Journal of Hydrogen Energy'' (2011) [http://dx.doi.org/10.1016/j.ijhydene.2011.03.172 doi:10.1016/j.ijhydene.2011.03.172] | *Smith PR, Bingham AS, Swartz JR. Generation of hydrogen from NADPH using an [FeFe] hydrogenase. ''International Journal of Hydrogen Energy'' (2011) [http://dx.doi.org/10.1016/j.ijhydene.2011.03.172 doi:10.1016/j.ijhydene.2011.03.172] | ||
Funding: We are grateful for funding from GCEP and the DOE [details] | Funding: We are grateful for funding from GCEP and the DOE [details] | ||
<html><iframe src="http://www.mendeley.com/groups/1295093/_/widget/29/5/" frameborder="0" allowTransparency="true" style="width:260px;height:550px;"></iframe><p style="width:260px"><a href="http://www.mendeley.com/groups/1295093/swartz-lab-biohydrogen/" title="Swartz Lab Biohydrogen on Mendeley">Swartz Lab Biohydrogen</a> is a group in <a href="http://www.mendeley.com/groups/engineering/" title="Engineering on Mendeley">Engineering</a> on <a href="http://www.mendeley.com/" title="Mendeley">Mendeley</a>.</p></html> | <html><iframe src="http://www.mendeley.com/groups/1295093/_/widget/29/5/" frameborder="0" allowTransparency="true" style="width:260px;height:550px;"></iframe><p style="width:260px"><a href="http://www.mendeley.com/groups/1295093/swartz-lab-biohydrogen/" title="Swartz Lab Biohydrogen on Mendeley">Swartz Lab Biohydrogen</a> is a group in <a href="http://www.mendeley.com/groups/engineering/" title="Engineering on Mendeley">Engineering</a> on <a href="http://www.mendeley.com/" title="Mendeley">Mendeley</a>.</p></html> | ||
Revision as of 12:43, 26 September 2011
The Biohydrogen team in the Swartz Lab focuses on developing Biotechnology-based solutions to the global energy problem. Specifically, we are interested in developing sustainable processes for the production of hydrogen from (1) Sunlight and (2) Biomass (see below).
The Biohydrogen team currently consists of Phil Smith, Alyssa Bingham, Kunal Mehta, Stacey Shiigi, and Sylvie Liong. Former members of the group include Marcus Boyer, James Stapleton, and Jon Kuchenreuter.
Early research by our team focused on building methods to produce active [FeFe] hydrogenases. These proteins catalyze the interconversion of protons, electrons, and dihydrogen; they are especially oxygen-sensitive due their FeS chemistry. [papers]
The first major goal of this research was the evolution of an oxygen-tolerant [FeFe] hydrogenase. This has proven to be extremely difficult; indeed many other research groups have tried this and been ultimately unsuccessful. However, our evolution methodology [papers] is different and has led to some level of success in this endeavor [paper]; however, much remains to be done to achieve this overall objective.
- Bingham AS, Smith PR, Swartz JR. Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.02.048
The original vision was that such a hydrogenase, once evolved, would be used in a photosynthetic microorganism to produce hydrogen directly from sunlight and water. This would require metabolic engineering of an insulated electron transfer circuit from the photosystems to the hydrogenase [papers, presentations]
A second project was begun in 2009 focusing on the production of hydrogen from biomass. The approach is to build a synthetic enzyme pathway that is able to produce hydrogen from NADPH; this pathway would then be coupled to the pentose phosphate pathway to enable hydrogen production from glucose (a proxy for biomass). [papers, presentations]
- Smith PR, Bingham AS, Swartz JR. Generation of hydrogen from NADPH using an [FeFe] hydrogenase. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.03.172
Funding: We are grateful for funding from GCEP and the DOE [details]
<html><iframe src="http://www.mendeley.com/groups/1295093/_/widget/29/5/" frameborder="0" allowTransparency="true" style="width:260px;height:550px;"></iframe><p style="width:260px"><a href="http://www.mendeley.com/groups/1295093/swartz-lab-biohydrogen/" title="Swartz Lab Biohydrogen on Mendeley">Swartz Lab Biohydrogen</a> is a group in <a href="http://www.mendeley.com/groups/engineering/" title="Engineering on Mendeley">Engineering</a> on <a href="http://www.mendeley.com/" title="Mendeley">Mendeley</a>.</p></html>
